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IHC (Immunohistochemistry)
(KRT19/CK19/Cytokeratin 19 Antibody-Anti-KRT19/CK19/Cytokeratin 19 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
Mouse KRT19/CK19/Cytokeratin 19 Monoclonal Antibody | anti-KRT19 antibody
KRT19/CK19/Cytokeratin 19 Mouse anti-Human Monoclonal (3F8) Antibody
Gene Names
KRT19; K19; CK19; K1CS
Reactivity
Dog, Rat, Human, Monkey
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence
Purity
Protein A/G purified
Synonyms
KRT19/CK19/Cytokeratin 19, Antibody; KRT19/CK19/Cytokeratin 19 Mouse anti-Human Monoclonal (3F8) Antibody; PathPlus KRT19/CK19/Cytokeratin 19 Antibody (clone 3F8); KRT19; CK19; Cytokeratin 19; Cytokeratin-19; Keratin 19; Keratin-19; CK-19; K19; K1CS; Keratin; type I; 40-kd; anti-KRT19 antibody
Host
Mouse
Reactivity
Dog, Rat, Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F8
Specificity
Human CK19/Cytokeratin 19
Purity/Purification
Protein A/G purified
Form/Format
PBS, pH7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Applicable Applications for anti-KRT19 antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence)
Target
Human KRT19/CK19/Cytokeratin 19
Immunogen
Recombinant fragment expressed in E Coli corresponding to amino acids 240-390 of human CK19.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze-thaw cycles.
IHC (Immunohistochemistry)
(KRT19/CK19/Cytokeratin 19 Antibody-Anti-KRT19/CK19/Cytokeratin 19 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
IHC (Immunohistochemistry)
(KRT19/CK19/Cytokeratin 19 Antibody-Anti-KRT19/CK19/Cytokeratin 19 antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)
WB (Western Blot)
(KRT19/CK19/Cytokeratin 19 Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19.)
WB (Western Blot)
(KRT19/CK19/Cytokeratin 19 Antibody-HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19.)
Application Data
(KRT19/CK19/Cytokeratin 19 Antibody-Western blot of extracts (35 ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.)
Application Data
(KRT19/CK19/Cytokeratin 19 Antibody-Western blot of extracts (35 ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.)
ICC (Immunocytochemistry)
(KRT19/CK19/Cytokeratin 19 Antibody-Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT19.)
ICC (Immunocytochemistry)
(KRT19/CK19/Cytokeratin 19 Antibody-Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT19.)
Related Product Information for anti-KRT19 antibody
Cytokeratin 19 antibody is an unconjugated mouse monoclonal antibody to Cytokeratin 19 (KRT19/CK19) from human. It is reactive with human, rat, dog and other species. Validated for IF, IHC and WB.
KRT19 is a type 1 keratin, an intermediate filament that provides structural support and has cell cycle regulatory functions in epithelial cells. KRT19 is a common epithelial marker with cytoplasmic positivity in immunohistochemistry. It is used to identify neoplastic epithelial cells in a number of cancers, including breast carcinomas, thyroid papillary carcinomas, cholangiocarcinomas, endometrial cancers, urothelial and collecting duct renal cancers, Paget's disease, pancreatic cancers, and epithelial odontogenic tumors. Additionally, it is a putative cancer stem cell marker in hepatocellular carcinoma. In normal tissue, KRT19 is positive in epithelia of the breast, GI tract, kidney, bile ducts, pancreatic ducts, salivary and sweat glands, prostate epithelium, placental trophoblasts, uterus, and tonsillar squamous epithelium.
KRT19 is a type 1 keratin, an intermediate filament that provides structural support and has cell cycle regulatory functions in epithelial cells. KRT19 is a common epithelial marker with cytoplasmic positivity in immunohistochemistry. It is used to identify neoplastic epithelial cells in a number of cancers, including breast carcinomas, thyroid papillary carcinomas, cholangiocarcinomas, endometrial cancers, urothelial and collecting duct renal cancers, Paget's disease, pancreatic cancers, and epithelial odontogenic tumors. Additionally, it is a putative cancer stem cell marker in hepatocellular carcinoma. In normal tissue, KRT19 is positive in epithelia of the breast, GI tract, kidney, bile ducts, pancreatic ducts, salivary and sweat glands, prostate epithelium, placental trophoblasts, uterus, and tonsillar squamous epithelium.
References
Clin Cancer Res. 2015 Jul 1;21(13):3081-91, PMID: 25820415; Int J Mol Sci. 2018 May; 19(5): 1423, PMID: 29747452; Cancers (Basel). 2019 Jan; 11(1): 99, PMID: 30650643
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,106 Da
NCBI Official Full Name
keratin, type I cytoskeletal 19
NCBI Official Synonym Full Names
keratin 19
NCBI Official Symbol
KRT19
NCBI Official Synonym Symbols
K19; CK19; K1CS
NCBI Protein Information
keratin, type I cytoskeletal 19; CK-19; keratin-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; 40-kDa keratin intermediate filament
UniProt Protein Name
Keratin, type I cytoskeletal 19
UniProt Gene Name
KRT19
UniProt Synonym Gene Names
CK-19; K19
UniProt Entry Name
K1C19_HUMAN
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Product Notes
The KRT19 krt19 (Catalog #AAA162600) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT19/CK19/Cytokeratin 19 Mouse anti-Human Monoclonal (3F8) Antibody reacts with Dog, Rat, Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's KRT19/CK19/Cytokeratin 19 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the KRT19 krt19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT19/CK19/Cytokeratin 19, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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