IF (Immunofluorescence)
(Immunofluorescence staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
Rabbit anti-Human L1CAM Monoclonal Antibody | anti-L1CAM antibody
Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Protein A
Synonyms
L1CAM, Antibody; Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal; CD171/N-CAML1/L1CAM Antibody, Rabbit MAb; L1 cell adhesion molecule; Anti-CAML1 Antibody; Anti-CD171 Antibody; Anti-HSAS Antibody; Anti-HSAS1 Antibody; Anti-MASA Antibody; Anti-MIC5 Antibody; Anti-N-CAM-L1 Antibody; Anti-N-CAML1 Antibody; Anti-NCAM-L1 Antibody; Anti-S10 Antibody; Anti-SPG1 Antibody; anti-L1CAM antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
014
Specificity
Human CD171/N-CAML1/L1CAM
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-L1CAM antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD171/N-CAML1/L1CAM protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD171/N-CAML1/L1CAM (rh CD171/N-CAML1/L1CAM).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to myenteric nerve plexus.)
IHC (Immunohistochemistry)
(Immunochemical staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to myenteric nerve plexus.)
Related Product Information for anti-L1CAM antibody
L1 cell adhesion molecule (L1CAM), also designated as CD171, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. While originally believed to be present only in brain cells, in recent years L1-CAM has been detected in other tissues, and a variety of cancer cells, including some common types of human cancer. L1CAM interacts with a variety of ligands including axonin-1, CD9, neurocan, and integrins, and it has been revealed that the RGD motif in the sixth Ig domain of L1CAM is a binding site for integrins, thus important for nuclear signaling. Disruption of L1CAM function causes three X-linked neurological syndromes, i.e. hydrocephalus, MASA syndrome (mental retardation, aphasia, shuffling gait, and adducted thumbs), and spastic paraplegia syndrome. Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate, and promoted cell transformation and tumorigenicity. Recent work has identified L1CAM (CD171) as a novel marker for human carcinoma progression, and a candidate for anti-cancer therapy.
References
Meier F, et al. (2006) The adhesion molecule L1 (CD171) promotes melanoma progression. Int J Cancer. 119(3): 549-55.Gavert N, et al. (2008) L1-CAM in cancerous tissues. Expert Opin Biol Ther. 8(11): 1749-57.Issa Y, et al. (2009) Enhanced L1CAM expression on pancreatic tumor endothelium mediates selective tumor cell transmigration. J Mol Med. 87(1): 99-112.Weidle UH, et al. (2009) L1-CAM as a target for treatment of cancer with monoclonal antibodies. Anticancer Res. 29(12): 4919-31.Raveh S, et al. (2009) L1 cell adhesion molecule (L1CAM) in invasive tumors. Cancer Lett. 282(2): 137-45.Wolterink S, et al. (2010) Therapeutic antibodies to human L1CAM: functional characterization and application in a mouse model for ovarian carcinoma. Cancer Res. 70(6): 2504-15.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,003 Da
NCBI Official Full Name
neural cell adhesion molecule L1 isoform 1
NCBI Official Synonym Full Names
L1 cell adhesion molecule
NCBI Official Symbol
L1CAM
NCBI Official Synonym Symbols
S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
NCBI Protein Information
neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
UniProt Protein Name
Neural cell adhesion molecule L1
UniProt Gene Name
L1CAM
UniProt Synonym Gene Names
CAML1; MIC5; N-CAM-L1; NCAM-L1
UniProt Entry Name
L1CAM_HUMAN
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Product Notes
The L1CAM l1cam (Catalog #AAA254552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-L1CAM Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's L1CAM can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the L1CAM l1cam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "L1CAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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