ICC (Immunocytochemistry)
(Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight 594.)
Mouse anti-Human L1CAM Monoclonal Antibody | anti-L1CAM antibody
L1CAM (Extracellular) Antibody
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Purity
Protein G chromatography
Synonyms
L1CAM, Antibody; L1CAM (Extracellular) Antibody; L1CAM (Extracellular); L1CAM M023; anti-L1CAM antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M023
Specificity
Clone M023 mouse monoclonal antibody detects a 230kDa* protein on SDS-PAGE immunoblots of human MeWo, MDA-MB-231, A431, and MCF7 cells, as well as human brain tissue. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects L1CAM on live cells.
Purity/Purification
Protein G chromatography
Form/Format
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-L1CAM antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Clone (M023) was generated from a proprietary antigen related to the native human L1CAM expressed in the MeWo melanoma cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight 594.)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of L1CAM in paraformaldehyde fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-L1CAM (LM0231). The antibody was detected using goat anti-mouse Ig DyLight 594.)
WB (Western Blot)
(Western blot analysis of human MDA-MB-231 cells (lane 1), MeWo cells (lane 2), human brain tissue (lane 3), A431 cells (lane 4), and MCF7 cells (lane 5). The blot was probed with mouse monoclonal anti-L1CAM (LM0231) at 1:1000.)
WB (Western Blot)
(Western blot analysis of human MDA-MB-231 cells (lane 1), MeWo cells (lane 2), human brain tissue (lane 3), A431 cells (lane 4), and MCF7 cells (lane 5). The blot was probed with mouse monoclonal anti-L1CAM (LM0231) at 1:1000.)
Related Product Information for anti-L1CAM antibody
The neural cell adhesion molecule L1 (L1CAM/NCAM-L1/CD171) is a single pass transmembrane glycoprotein member of the immunoglobulin superfamily that contains six Ig-like domains and five fibronectin type-III domains in the extracellular region. L1CAM is mainly expressed in the brain where it has important roles in the developing nervous system. L1CAM has been implicated in neuronal migration and adhesion, axon myelination and fasciculation, and neurite outgrowth. Mutations in the L1CAM gene cause varying degrees of neurological disease including X-linked hydrocephalus, MASA syndrome, spastic paraplegia type 1, and X-linked corpus callosum agenesis. Apart from the nervous system, L1CAM is overexpressed in many cancers and supports a poor prognosis by facilitating aggressive tumor growth, metastasis, and chemoresistance.
References
Angiolini F, Cavallaro U (2017) Int J Mol Sci 18(2)pii:E254.
Hortsch, M. (1996) Neuron. 17:587.
Moos, M. et al. (1988) Nature. 334:701.
Hortsch, M. (1996) Neuron. 17:587.
Moos, M. et al. (1988) Nature. 334:701.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
140,003 Da
NCBI Official Full Name
neural cell adhesion molecule L1 isoform 1
NCBI Official Synonym Full Names
L1 cell adhesion molecule
NCBI Official Symbol
L1CAM
NCBI Official Synonym Symbols
S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
NCBI Protein Information
neural cell adhesion molecule L1; antigen identified by monoclonal antibody R1
UniProt Protein Name
Neural cell adhesion molecule L1
UniProt Gene Name
L1CAM
UniProt Synonym Gene Names
CAML1; MIC5; N-CAM-L1; NCAM-L1
UniProt Entry Name
L1CAM_HUMAN
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Product Notes
The L1CAM l1cam (Catalog #AAA71655) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The L1CAM (Extracellular) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's L1CAM can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the L1CAM l1cam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "L1CAM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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