FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293 transfected cells stained with AAA243697 (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse lacZ Monoclonal Antibody | anti-lacZ antibody
lacZ Monoclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293 transfected cells stained with AAA243697 (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay histogram showing 293 transfected cells stained with AAA243697 (red line) at 1:200. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
IP (Immunoprecipitation)
(Immunoprecipitating lacZ in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA243697 in 293F transfected whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA243697 (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IP (Immunoprecipitation)
(Immunoprecipitating lacZ in 293F transfected whole cell lysateLane 1: Mouse control IgG (1ug) instead of AAA243697 in 293F transfected whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA243697 (5ug) + 293F transfected whole cell lysate (500ug)Lane 3: 293F transfected whole cell lysate (20ug))
IF (Immunofluorescence)
(Immunofluorescence staining of 293 transfected cells with AAA243697 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of 293 transfected cells with AAA243697 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)All lanes: lacZ antibody at 1:2000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant protein (10ng, 5ng, 2.5ng, 1.25ng, 0.625ng)All lanes: lacZ antibody at 1:2000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: lacZ antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000SecondaryGoat polyclonal to Mouse IgG at 1/50000 dilutionPredicted band size: 117 kDaObserved band size: 130 kDa)
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Product Notes
The lacZ lacz (Catalog #AAA243697) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The lacZ Monoclonal Antibody reacts with Escherichia coli and may cross-react with other species as described in the data sheet. AAA Biotech's lacZ can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the lacZ lacz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "lacZ, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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