FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA278885 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
Mouse anti-Human LAG3 Monoclonal Antibody | anti-LAG3 antibody
LAG3 Monoclonal Antibody
Gene Names
LAG3; CD223
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, ELISA
Purity
>95%, Protein G purified
Synonyms
LAG3, Antibody; LAG3 Monoclonal Antibody; CD223 antibody; CD223 antigen antibody; FDC protein antibody; LAG-3 antibody; Lag3 antibody; LAG3_HUMAN antibody; Lymphocyte activating 3 antibody; Lymphocyte activation gene 3 antibody; Lymphocyte activation gene 3 protein antibody; Protein FDC antibody; anti-LAG3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
8B12E10
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-LAG3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human Lymphocyte activation gene 3 protein (29-450AA)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA278885 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing A549 cells stained with AAA278885 (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IHC (Immunohiostchemistry)
(IHC image of AAA278885 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image of AAA278885 diluted at 1:500 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37 degree C Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: 1 lane: Recombinant proteins with LAG3; 2 lane: 293F whole cell lysate transfected with LAG3All lanes: LAG3 antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 76, 88 KDaObserved band size: 76, 88 KDaExposure time?5min)
WB (Western Blot)
(Western BlotPositive WB detected in: 1 lane: Recombinant proteins with LAG3; 2 lane: 293F whole cell lysate transfected with LAG3All lanes: LAG3 antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 76, 88 KDaObserved band size: 76, 88 KDaExposure time?5min)
Product Categories/Family for anti-LAG3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
525
NCBI Official Full Name
Lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte-activation gene 3
NCBI Official Symbol
LAG3
NCBI Official Synonym Symbols
CD223
NCBI Protein Information
lymphocyte activation gene 3 protein
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Gene Name
LAG3
UniProt Synonym Gene Names
FDC; LAG-3
UniProt Entry Name
LAG3_HUMAN
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Product Notes
The LAG3 lag3 (Catalog #AAA278885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAG3 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAG3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LAG3 lag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAG3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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