ELISA
(Binding curve of anti-Lag3 antibody C9B7W to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein at a concentration of 2ug/ml. A 4-fold serial dilution from 5,000ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Rat anti-Mouse Lag3 Monoclonal Antibody | anti-Lag3 antibody
Anti-Lag3 [C9B7W], Rat IgG1, Kappa
Gene Names
Lag3; Ly66; CD223; LAG-3
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
Protein A affinity purified
Synonyms
Lag3, Antibody; Anti-Lag3 [C9B7W], Rat IgG1, Kappa; CD223; LAG-3; Lymphocyte activation gene 3 protein; anti-Lag3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1, Kappa
Clone Number
C9B7W
Specificity
The antibody is specific for an epitope in the D2 domain of murine LAG-3.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Lag3 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), Blocking
Antibody Type
Recombinant Antibody
Immunogen
This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA
(Binding curve of anti-Lag3 antibody C9B7W to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein at a concentration of 2ug/ml. A 4-fold serial dilution from 5,000ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
ELISA
(Binding curve of anti-Lag3 antibody C9B7W to recombinant mouse Lag3 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse Lag3 Fc-Fusion Protein at a concentration of 2ug/ml. A 4-fold serial dilution from 5,000ng/ml was performed. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody C9B7W Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of C9B7W at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse splenocytes using anti-Lag3 antibody C9B7W Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of C9B7W at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-Lag3 antibody
Describes the original generation of this antibody, and its characterisation.
Recombinant monoclonal antibody to Lag3. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C9B7W.
Recombinant monoclonal antibody to Lag3. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma C9B7W.
Product Categories/Family for anti-Lag3 antibody
References
Workman et al.; Eur J Immunol. 2002 Aug;32(8):2255-63
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,978 Da
NCBI Official Full Name
lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte-activation gene 3
NCBI Official Symbol
Lag3
NCBI Official Synonym Symbols
Ly66; CD223; LAG-3
NCBI Protein Information
lymphocyte activation gene 3 protein
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Gene Name
Lag3
UniProt Synonym Gene Names
LAG-3
UniProt Entry Name
LAG3_MOUSE
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Product Notes
The Lag3 lag3 (Catalog #AAA72063) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Lag3 [C9B7W], Rat IgG1, Kappa reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lag3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), Blocking. Researchers should empirically determine the suitability of the Lag3 lag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lag3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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