Rabbit Lamin A/C Monoclonal Antibody

[KO Validated] Lamin A/C Rabbit mAb

Cat. Num. AAA28306

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: Lamin A/C, Antibody; [KO Validated] Lamin A/C Rabbit mAb; LMNA; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNC; LMNL1; MADA; PRO1; lamin; anti-Lamin A/C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification

• Applicable Applications for anti-Lamin A/C antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB 1:50000 - 1:200000; IHC 1:50 - 1:200; IF 1:50 - 1:200; IP 1:50 - 1:200
• Immunogen: Recombinant protein of human Lamin A/C.
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Positive Samples: K-562, A-549, NIH/3T3, Rat lung, HeLa, HeLa whole cell protein, HeLa cytoplasm protein, HeLa nuclear protein, NIH/3T3 whole cell protein, NIH/3T3 cytoplasm protein, NIH/3T3 nuclear protein, C6 whole cell protein, C6 cytoplasm protein, C6 nuclear protein
• Cellular Location: Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A/C antibody. Western blot was performed from the immunoprecipitate using Lamin A/C antibody at a dilition of 1:20000.)

IF (Immunofluorescence)

(Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of L929 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody at dilution of 1:200 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)

WB (Western Blot)

(Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)

Related Product Information for anti-Lamin A/C antibody

The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.

NCBI and Uniprot Product Information

• UniProt Accession #: P02545
• Molecular Weight: Calculated MW 62-74kDa; Observed MW 62kDa/74kDa