Mouse Lamin A+C Monoclonal Antibody | anti-LMNA antibody

Lamin A+C Monoclonal Antibody [JOL2]

Cat. Num. AAA13582

• Gene Names: LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• Reactivity: Human, African Green Monkey
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Unpurified
• Synonyms: Lamin A+C, Antibody; Lamin A+C Monoclonal Antibody [JOL2]; anti-LMNA antibody

• Host: Mouse
• Reactivity: Human, African Green Monkey
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: JOL2
• Purity/Purification: Unpurified
• Form/Format: Purified antibody (from supernatant) containing PBS + 0.09% sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LMNA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000; IF: 1:100; FC/FACS: 1:1000
• Immunogen: Recombinant human lamin A
• Target Antigen: This antibody reacts with both recombinant and native Lamin A and C in humans
• Myeloma/Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2-0/Ag4 myeloma cell line
• Pubmed ID: 30650545, 31048846, 30422822, 31847370, 30126195, 29405587, 28668644, 29156644, 28985428, 28031327, 28179995, 27378237, 27031510, 27926867, 26124276, 26447202, 24452336, 24659496, 24838313, 24950247, 24732130, 23975040, 22871573, 22253444, 21610090, 21852285, 21392397, 22040608, 19623164, 11207047

Application Data

(Lamin A+C Monoclonal Antibody [JOL2])

IHC (Immunohistchemistry)

(IHC using Lamin A/C antibody (IQ332) clone JOL2 on Human colon)

IB (Immunoblot)

(Immunoblotting of Hela cells with Lamin A+C Antibody (IQ332) clone JOL2)

IHC (Immunohistochemistry)

(IHC using Lamin A/C Antibody (IQ332) clone JOL2 on Human skin)

Application Data

((1:1000) staining in A431(A), (1:10000) staining in HeLa(B), and (1:1000) staining in HepG2(C) cells nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

FCM (Flow Cytometry)

(Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor 488 secondary antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 383792150
• NCBI GeneID: 4000
• NCBI Accession #: NP_001244303.1
• NCBI GenBank Nucleotide #: NM_001257374.1
• UniProt Accession #: P02545; P02546; Q5TCJ2; Q5TCJ3; Q969I8; Q96JA2; B4DI32; D3DVB0; E7EUI9
• Molecular Weight: 74,65kDa
• NCBI Official Full Name: lamin isoform D
• NCBI Official Synonym Full Names: lamin A/C
• NCBI Official Symbol: LMNA
• NCBI Official Synonym Symbols: FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
• NCBI Protein Information: lamin; 70 kDa lamin; prelamin-A/C; lamin A/C-like 1; renal carcinoma antigen NY-REN-32
• UniProt Protein Name: Prelamin-A/C
• UniProt Gene Name: LMNA
• UniProt Synonym Gene Names: LMN1
• UniProt Entry Name: LMNA_HUMAN