Mouse Lamin B2 Monoclonal Antibody | anti-LMNB2 antibody

Mouse anti Lamin B2

Cat. Num. AAA77480

• Gene Names: LMNB2; LMN2; LAMB2; MGC2721
• Reactivity: Hamster, Human, Mouse, Swine, Xenopus, Zebrafish
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: Lamin B2, Antibody; Mouse anti Lamin B2; anti-LMNB2 antibody

• Host: Mouse
• Reactivity: Hamster, Human, Mouse, Swine, Xenopus, Zebrafish
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: LN43
• Specificity: LN43 reacts with an epitope loCated in the C-terminal part of lamin B2.
• Form/Format: Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.

• Applicable Applications for anti-LMNB2 antibody: WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
• Source Note: LN43 is a Mouse monoclonal IgG1 antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with the detergent insoluble fraction of potoroo cell line PtK1.
• Preparation and Storage: Store at 4 degree C, or in small aliquots at -20 degree C.

IHC (Immunohistochemisry)

(Figure 3. immunohistochemistry on a frozen section of human kidney showing nuclear lamina staining in the ductal epithelium.)

IF (Immunofluorescence)

(Figure 2. immunofluorescence staining of a 9 days old zebrafish embryo.)

IHC (Immunohistochemistry)

(Figure 1. immunohistochemistry on frozen sections of human kidney showing nuclear lamina staining in the ductal epithelium.)

Related Product Information for anti-LMNB2 antibody

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, ie lamin B1 and lamin B2.

Product Categories/Family for anti-LMNB2 antibody

Nuclear proteins; Cytoskeleton

References

Bridger, J. M., Kill, I. R., O'Farrell, M., and Hutchison, C. J. (1993). Internal lamin structures within G1 nuclei of Human dermal fibroblasts, J Cell Sci 104, 297-306. Jenkins, H., Hölman, T., Lyon, C., Lane, B., Stick, R., and Hutchison, C. J. (1993). Nuclei that lack a lamina accumulate karyophilic proteins and assemble a nuclear matrix, J Cell Sci 106, 275-285. Hozak, P., Sasseville, A. M., Raymond, Y., and Cook, P. R. (1995). Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in Human cells, J Cell Sci 108, 635-44. Machiels, B. M., Broers, J. L., Raymond, Y., de Ley, L., Kuijpers, H. J., Caberg, N. E., and Ramaekers, F. C. (1995). Abnormal A-type lamin organization in a Human lung carcinoma cell line, Eur J Cell Biol 67, 328-35. Machiels, B. M., Zorenc, A. H., Endert, J. M., Kuijpers, H. J., van Eys, G. J., Ramaekers, F. C., and Broers, J. L. (1996). An alternative splicing product of the lamin A/C gene lacks exon 10, J Biol Chem 271, 9249-53. Machiels, B. M., Ramaekers, F. C., Kuijpers, H. J., Groenewoud, J. S., Oosterhuis, J. W., and Looijenga, L. H. (1997). Nuclear lamin expression in normal testis and testicular germ cell tumours of adolescents and adults, J Pathol 182, 197-204. Jansen, M. P., Machiels, B. M., Hopman, A. H., Broers, J. L., Bot, F. J., Arends, J. W., Ramaekers, F. C., and Schouten, H. C. (1997). Comparison of A and B-type lamin expression in reactive lymph nodes and nodular sclerosing Hodgkin's disease, Histopathology 31, 304-12. Broers, J. L., Machiels, B. M., Kuijpers, H. J., Smedts, F., van den Kieboom, R., Raymond, Y., and Ramaekers, F. C. (1997). A- and B-type lamins are differentially expressed in normal Human tissues, Histochem Cell Biol 107, 505-17.

NCBI and Uniprot Product Information

• NCBI GI #: 1054874
• NCBI GeneID: 84823
• UniProt Accession #: Q03252; O75292; Q14734; Q96DF6
• Molecular Weight: 67,689 Da
• NCBI Official Full Name: lamin B2
• NCBI Official Synonym Full Names: lamin B2
• NCBI Official Symbol: LMNB2
• NCBI Official Synonym Symbols: LMN2; LAMB2; MGC2721
• NCBI Protein Information: lamin-B2; OTTHUMP00000198023
• UniProt Protein Name: Lamin-B2
• UniProt Gene Name: LMNB2
• UniProt Synonym Gene Names: LMN2
• UniProt Entry Name: LMNB2_HUMAN