Mouse anti-Human Laminin beta2/gamma1 Subunits Monoclonal Antibody | anti-LAMC1 antibody

Laminin beta2/gamma1 Subunits Antibody

Cat. Num. AAA71656

• Gene Names: LAMC1; LAMB2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Protein G chromatography
• Synonyms: Laminin beta2/gamma1 Subunits, Antibody; Laminin beta2/gamma1 Subunits Antibody; Laminin b2; anti-LAMC1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: M046
• Specificity: Clone M046 detects native laminin isoforms that include both laminin beta2 and gamma1 subunits. In native immunoblots, the antibody detects laminin 521, laminin 221, and laminin 121, but does not detect lamin 332 and laminin 411. The antibody weakly detects laminin 511. This antibody can be used for immunocytochemical detection of laminin beta2/gamma1 subunits, and can also be used for detection of native laminin isoforms in immunoblots and ELISA.
• Purity/Purification: Protein G chromatography
• Form/Format: Mouse monoclonal antibody purified with protein G chromatography is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.

• Applicable Applications for anti-LAMC1 antibody: ELISA, WB (Western Blot), ICC (Immunocytochemistry)
• Immunogen: Clone (M046) was generated from a recombinant human laminin 521 protein that includes a heterotrimer of three subunits laminin alpha5, laminin beta2, and laminin gamma1 subunits.
• Preparation and Storage: Store at -20 degree C. Stable for 1 year.

ICC (Immunocytochemistry)

(Immunocytochemical labeling of laminin beta2/gamma1 subunits in aldehyde fixed and NP-40 permeabilized human MDA-MB-231 breast carcinoma cells. The cells were labeled with mouse monoclonal anti-Laminin beta2/gamma1 subunits (LM0461). The antibody was detected using goat anti-mouse DyLight 594.)

WB (Western Blot)

(Native western blot image of human laminin isoforms: laminin 521 (alpha5beta2gamma1), laminin 121 (alpha1beta2gamma1), laminin 221 (alpha2beta2gamma1), laminin 332 (alpha3beta3gamma2), laminin 511 (alpha5beta1gamma1), laminin 411 (alpha4beta1gamma1), as well as human A431, A549, and NCI-H2052 cells. The blot was probed with mouse monoclonal anti-Laminin beta2/gamma1 subunit (LM0461) at 1:1000.)

Related Product Information for anti-LAMC1 antibody

The human basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850kDa) oligomer, consisting of three different chains laminin alpha (alpha), beta (beta), and gamma (gamma) joined by disulfide bonds. The structure of human laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain including 5 alpha chains, 4 beta chains, and 3 gamma chains. Laminin beta2 and gamma1 are found in laminin 121, laminin 221, laminin 421, and laminin 521. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.

References

Hall ZW. (1995) Science. 269(5222):362-3.
Aumailley M. (2013) Cell Adh Migr. 7(1):48-55.

NCBI and Uniprot Product Information

• NCBI GI #: 145309326
• NCBI GeneID: 3915
• NCBI Accession #: NP_002284.3
• NCBI GenBank Nucleotide #: NM_002293.3
• UniProt Accession #: P11047; Q5VYE7
• Molecular Weight: 177,603 Da
• NCBI Official Full Name: laminin subunit gamma-1
• NCBI Official Synonym Full Names: laminin, gamma 1 (formerly LAMB2)
• NCBI Official Symbol: LAMC1
• NCBI Official Synonym Symbols: LAMB2
• NCBI Protein Information: laminin subunit gamma-1; S-LAM gamma; laminin B2 chain; S-laminin subunit gamma; laminin-2 subunit gamma; laminin-3 subunit gamma; laminin-4 subunit gamma; laminin-6 subunit gamma; laminin-7 subunit gamma; laminin-8 subunit gamma; laminin-9 subunit gamma
• UniProt Protein Name: Laminin subunit gamma-1
• UniProt Gene Name: LAMC1
• UniProt Synonym Gene Names: LAMB2; S-LAM gamma
• UniProt Entry Name: LAMC1_HUMAN