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WB (Western Blot)
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Lasu1/Ureb1 recombinant antibody

Rabbit anti-Lasu1/Ureb1 Recombinant Monoclonal Antibody [BLR129H]

Gene Names
HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
Lasu1/Ureb1, Recombinant Antibody; Rabbit anti-Lasu1/Ureb1 Recombinant Monoclonal Antibody [BLR129H]; large structure of UREB1; URE-binding protein 1; LASU1; HECT, UBA and WWE domain-containing protein 1; Mcl-1 ubiquitin ligase E3; MULE; upstream regulatory element-binding protein 1; URE-B1; Ib772; UREB1; HSPC272; homologous to E6AP carboxyl terminus homologous protein 9; HECTH9; HECT domain protein LASU1; E3 ubiquitin-protein ligase HUWE1; BJ-HCC-24 tumor antigen; ARF-BP1; ARF-binding protein 1; HECT-type E3 ubiquitin transferase HUWE1; HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase; Lasu1/Ureb1 recombinant antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR129H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
10ug/ml (varies by lot)
Applicable Applications for Lasu1/Ureb1 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000 (3-8% Tris-acetate gel recommended)
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 2275 and 2325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (20 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot)

(Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (2 ug) from GaMG, K-562, Jurkat, Hep-G2, LNCaP, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used for IP at 20 ul/mg lysate. Lasu1/Ureb1 was also immunoprecipitated by other Lasu1/Ureb1 antibodies and BL672. For blotting immunoprecipitated Lasu1-Ureb1, AAA23842 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of human Lasu1/Ureb1 in FFPE head and neck squamous cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry)

(Detection of human Lasu1/Ureb1 in FFPE SiHa cells by immunocytochemistry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry)

(Detection of human Lasu1/Ureb1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

Related Product Information for Lasu1/Ureb1 recombinant antibody
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Lasu1/Ureb1 is an E3 ubiquitin protein ligase that mediates the ubiquitination of core histones, Mcl-1, Cdc6, and p53. Lasu1/Ureb1 expression is regulated during spermatogenesis and appears to play a role in chromatin modification in germ cells as well as other tissues.
Product Categories/Family for Lasu1/Ureb1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
481,018 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase HUWE1
NCBI Official Synonym Full Names
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
NCBI Official Symbol
HUWE1
NCBI Official Synonym Symbols
MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
NCBI Protein Information
E3 ubiquitin-protein ligase HUWE1; ARF-binding protein 1; BJ-HCC-24 tumor antigen; HECT domain protein LASU1; Mcl-1 ubiquitin ligase E3; URE-binding protein 1; homologous to E6AP carboxyl terminus homologous protein 9; large structure of UREB1; upstream r
UniProt Protein Name
E3 ubiquitin-protein ligase HUWE1
UniProt Gene Name
HUWE1
UniProt Synonym Gene Names
KIAA0312; KIAA1578; UREB1; ARF-BP1; HectH9; LASU1; Mule; URE-B1; URE-binding protein 1
UniProt Entry Name
HUWE1_HUMAN

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product-image-AAA23842_WB6.jpg

Lasu1/Ureb1 Recombinant Antibody

Host: Rabbit
Reactivity: Human, Mouse
Apps: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay

Product Notes

The Lasu1/Ureb1 huwe1 (Catalog #AAA23842) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Lasu1/Ureb1 Recombinant Monoclonal Antibody [BLR129H] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lasu1/Ureb1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 (3-8% Tris-acetate gel recommended) IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the Lasu1/Ureb1 huwe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lasu1/Ureb1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.