IP (Immunoprecipitation)
(Detection of human Desmoplakin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Desmoplakin antibody AAA212155 used for IP at 6 ug/mg lysate. Desmoplakin was also immunoprecipitated by rabbit anti-Desmoplakin antibody which recognizes an upstream epitope. For blotting immunoprecipitated Desmoplakin, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human Desmoplakin Polyclonal Antibody | anti-DSP antibody
Rabbit anti-Desmoplakin Antibody, Affinity Purified
Gene Names
DSP; DP; DPI; DPII; DCWHKTA
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Desmoplakin, Antibody; Rabbit anti-Desmoplakin Antibody, Affinity Purified; DP; desmoplakin; 250/210 kDa paraneoplastic pemphigus antigen; DCWHKTA; Desmoplakin; anti-DSP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DSP antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1925 and 1975
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Desmoplakin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Desmoplakin antibody AAA212155 used for IP at 6 ug/mg lysate. Desmoplakin was also immunoprecipitated by rabbit anti-Desmoplakin antibody which recognizes an upstream epitope. For blotting immunoprecipitated Desmoplakin, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Desmoplakin by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Desmoplakin antibody AAA212155 used for IP at 6 ug/mg lysate. Desmoplakin was also immunoprecipitated by rabbit anti-Desmoplakin antibody which recognizes an upstream epitope. For blotting immunoprecipitated Desmoplakin, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Desmoplakin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Desmoplakin (Cat. No. AAA212155 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Desmoplakin by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Desmoplakin (Cat. No. AAA212155 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-DSP antibody
Desmosomes are intercellular junctions that tightly link adjacent cells. Desmoplakin is an obligate component of functional desmosomes that anchors intermediate filaments to desmosomal plaques. The N-terminus of desmoplakin is required for localization to the desmosome and interacts with the N-terminal region of plakophilin 1 and plakoglobin. The C-terminus of desmoplakin binds with intermediate filaments. In the mid-region of desmoplakin, a coiled-coiled rod domain is responsible for homodimerization. Mutations in DSP, the gene encoding desmoplakin, are the cause of several cardiomyopathies and keratodermas as well as the autoimmune disease paraneoplastic pemphigus [taken from NCBI Entrez Gene (Gene ID: 1832)].
Product Categories/Family for anti-DSP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
278,916 Da
NCBI Official Full Name
desmoplakin isoform I
NCBI Official Synonym Full Names
desmoplakin
NCBI Official Symbol
DSP
NCBI Official Synonym Symbols
DP; DPI; DPII; DCWHKTA
NCBI Protein Information
desmoplakin; desmoplakin I; desmoplakin II; 250/210 kDa paraneoplastic pemphigus antigen
UniProt Protein Name
Desmoplakin
UniProt Gene Name
DSP
UniProt Synonym Gene Names
DP
UniProt Entry Name
DESP_HUMAN
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Product Notes
The DSP dsp (Catalog #AAA212155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Desmoplakin Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Desmoplakin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the DSP dsp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Desmoplakin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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