IP (Immunoprecipitation)
(LXN was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-LXN mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LXN mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDaObserved band size: 27 kDa)
Mouse anti-Human Latexin Monoclonal Antibody | anti-LXN antibody
Anti-Latexin Antibody, Mouse Monoclonal
Gene Names
LXN; ECI; TCI
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
Latexin, Antibody; Anti-Latexin Antibody, Mouse Monoclonal; Latexin/LXN/TCI Antibody, Mouse MAb; latexin; Anti-ECI Antibody; Anti-TCI Antibody; anti-LXN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
8E1G6C1D4
Specificity
Human Latexin/LXN/TCI.
No cross-reactivity in ELISA with E Coli lysate
No cross-reactivity in ELISA with E Coli lysate
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-LXN antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human Latexin protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Latexin (rh Latexin). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(LXN was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-LXN mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LXN mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDaObserved band size: 27 kDa)
IP (Immunoprecipitation)
(LXN was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-LXN mouse monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LXN mouse monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDaObserved band size: 27 kDa)
WB (Western Blot)
(Anti-LXN mouse monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)
WB (Western Blot)
(Anti-LXN mouse monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)
Related Product Information for anti-LXN antibody
Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin/LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.
References
Liu Q., et al., (2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.Muzny D.M., et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,750 Da
NCBI Official Full Name
latexin
NCBI Official Synonym Full Names
latexin
NCBI Official Symbol
LXN
NCBI Official Synonym Symbols
ECI; TCI
NCBI Protein Information
latexin; MUM; endogenous carboxypeptidase inhibitor; tissue carboxypeptidase inhibitor
UniProt Protein Name
Latexin
UniProt Gene Name
LXN
UniProt Synonym Gene Names
ECI; TCI
UniProt Entry Name
LXN_HUMAN
Similar Products
Product Notes
The LXN lxn (Catalog #AAA254706) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Latexin Antibody, Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Latexin can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LXN lxn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Latexin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.