SDS-PAGE
(The cell lysate of A549 (40ug) was resolved by SDS-PAGE, transferred to PVDF, membrane and probed with anti-human LAYN antibody (1:3000). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system)
Mouse anti-Human LAYN Monoclonal Antibody | anti-LAYN antibody
LAYN antibody
Reactivity
Human
Applications
Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
LAYN, Antibody; LAYN antibody; Layilin isoform 1; Layilin; anti-LAYN antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT20G8
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
382
Applicable Applications for anti-LAYN antibody
WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human LAYN (22-235aa) purified from E. coli
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(The cell lysate of A549 (40ug) was resolved by SDS-PAGE, transferred to PVDF, membrane and probed with anti-human LAYN antibody (1:3000). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system)
SDS-PAGE
(The cell lysate of A549 (40ug) was resolved by SDS-PAGE, transferred to PVDF, membrane and probed with anti-human LAYN antibody (1:3000). Protein were visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system)
Related Product Information for anti-LAYN antibody
Layilin is a widely expressed integral membrane hyaluronan receptor and a transmembrane protein with homology to C-type lectins. This protein interacts with the ERM protein family of cytoskeletal membrane linker molecule members, including talin, ezrin, moesin, and radixin. The carboxy-terminal domain of layilin is capable of binding to the amino-terminal domain of radixin. An interdomain interaction between the amino- and the carboxy-terminal domains of radixin inhibits its ability to bind to layilin. Layilin plays an important role in cell adhesion and motility. It is important for the maintenance of the cortical structure in the cell because it mediates signals from the extracellular matrix to the cytoskeleton. In spreading cells, Layilin acts as a membrane-binding site for talin in the peripheral ruffles, and it may play a role in the migration of cells to a wound site.
Product Categories/Family for anti-LAYN antibody
References
Bono P., et al. (2005) Exp Cell Res 1;308 (1): 177-87; Mark L., et al. (1998) The Journal of Cell Biology 143 (2): 429;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
layilin isoform 1
NCBI Official Synonym Full Names
layilin
NCBI Official Symbol
LAYN
NCBI Protein Information
layilin
UniProt Protein Name
Layilin
UniProt Gene Name
LAYN
Similar Products
Product Notes
The LAYN layn (Catalog #AAA48138) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAYN antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAYN can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LAYN layn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAYN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.