Mouse Leishmania A2 Monoclonal Antibody

Leishmania A2 Mouse Monoclonal Antibody (C9)

Cat. Num. AAA77830

• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Synonyms: Leishmania A2, Antibody; Leishmania A2 Mouse Monoclonal Antibody (C9); anti-Leishmania A2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: C9
• Specificity: A sensitive antibody to Leishmania amastigote-specific A2 protein. This sequence can also be used as tag if cloned with the protein of interest. This sequence is particular to the parasite and therefore the antibody has no crossreactivity with mammalian proteins. It is specific to A2 protein from Leishamania donovani and in a highly probable manner to other species which express A2. It won't be detected in species where the A2 is non-expressed pseudogenes such as Leishmania major.
• Form/Format: Lyophilized protein G purified in PBS pH7.4

• Applicable Applications for anti-Leishmania A2 antibody: WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence)
• Immunogen: The anti-A2 antibody recognizes only the amastigote form of the A2 protein. The anti-A2 antibody recognizes the sequence QSVGPLSVGP from the parasite, Leishmania donovani.
• Target: Leishmania A2
• Preparation and Storage: Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Related Product Information for anti-Leishmania A2 antibody

Leishmania donovani is a protozoa parasite responsible for human visceral leishmaniasis. During their life cycle the parasites exist in 2 forms: as flagellated promastigote within the sandfly vector and as non-flagellated amastigote in the macrophage phagolysosomal compartment of the mammalian host. A2 protein is an amastigote-specific virulence factor that is required for Leishmania donovani parasite survival in a mammalian host.

References

1. Jaiswal AK-Immunological consequences of stress-related proteins-cytosolic tryparedoxin peroxidase and chaperonin TCP20--identified in splenic amastigotes. 2. Kumari S-Leishmania donovani: immunostimulatory cellular responses of membrane and soluble protein fractions of splenic amastigotes in cured patient and ham. 3. McCall LI and Matlashewski G-Localization and induction of the A2 virulence factor in Leishmania: evidence that A2 is a stress response protein. 4. Nascimento M-Identification and characterization of a protein-tyrosine phosphatase in Leishmania: Involvement in virulence. 5. Zhang WW-Comparison of the A2 gene locus in Leishmania donovani and Leishmania major and its control over cutaneous infection. 6. Carvalho FA-Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen. 7. Zhang WW and Matlashewski G-Loss of virulence in Leishmania donovani deficient in an amastigote-specific protein, A2.