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ELISA
(Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.)
Mouse anti-Human LIPA/Lysosomal Acid Lipase Monoclonal Antibody | anti-LIPA antibody
Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9)
Gene Names
LIPA; LAL; CESD
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, ELISA
Purity
Purified from ascites by Protein A
Synonyms
LIPA/Lysosomal Acid Lipase, Antibody; Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9); Rabbit Polyclonal to Human LIPA/Lysosomal Acid Lipase; LIPA; Cholesterol ester hydrolase; Cholesteryl esterase; LAL; Lysosomal acid lipase; Lipase A; Sterol esterase; CESD; anti-LIPA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
1F9
Specificity
Human LIPA/Lysosomal Acid Lipase
Purity/Purification
Purified from ascites by Protein A
Form/Format
PBS, pH 7.4.
Sequence Length
399
Applicable Applications for anti-LIPA antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA
Immunogen
LIPA (AAH12287, aa1-399 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C or lower.
Aliquot to avoid repeated freezing and thawing.
Aliquot to avoid repeated freezing and thawing.
ELISA
(Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.)
ELISA
(Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.)
WB (Western Blot)
(Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot)
(Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate(45 KDa).Lane 2: Non-transfected lysate.)
IHC (Immunohistochemistry)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
Related Product Information for anti-LIPA antibody
intracellular hydrolysis of cholesteryl esters and triglycerides thathave been internalized via receptor-mediated endocytosis of lipoproteinparticles. Important in mediating the effect of LDL (low densitylipoprotein) uptake on suppression of hydroxymethylglutaryl-CoAreductase and activation of endogenous cellular cholesteryl esterformation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,112 Da
NCBI Official Full Name
lysosomal acid lipase/cholesteryl ester hydrolase isoform 1
NCBI Official Synonym Full Names
lipase A, lysosomal acid type
NCBI Official Symbol
LIPA
NCBI Official Synonym Symbols
LAL; CESD
NCBI Protein Information
lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Protein Name
Lysosomal acid lipase/cholesteryl ester hydrolase
UniProt Gene Name
LIPA
UniProt Synonym Gene Names
Acid cholesteryl ester hydrolase; LAL
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Product Notes
The LIPA lipa (Catalog #AAA162318) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-LIPA/Lysosomal Acid Lipase Antibody (clone 1F9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIPA/Lysosomal Acid Lipase can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the LIPA lipa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPA/Lysosomal Acid Lipase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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