IF (Immunofluorescence)
(Immunofluorescence analysis of HEK293T cells transfected with Luciferase . 1.Luciferase Mouse Monoclonal Antibody(6B8)(green) was diluted at 1:200(4 degree C.overnight). 2. Dylight 488 labled Secondary antibody was diluted at 1:300 room temperature. 30min(Assay Biotech SA0403). DAPI(blue) 10min.)
Mouse anti-Firefly Luciferase Monoclonal Antibody | anti-Luciferase antibody
Luciferase Mouse Monoclonal Antibody (6B8)
Reactivity
Firefly
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Luciferase, Antibody; Luciferase Mouse Monoclonal Antibody (6B8); Luciferase; anti-Luciferase antibody
Host
Mouse
Reactivity
Firefly
Clonality
Monoclonal
Clone Number
6B8
Specificity
Luciferase protein detects endogenous levels of Luciferase
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Luciferase antibody
WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
Luciferase protein detects endogenous levels of Luciferase
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of HEK293T cells transfected with Luciferase . 1.Luciferase Mouse Monoclonal Antibody(6B8)(green) was diluted at 1:200(4 degree C.overnight). 2. Dylight 488 labled Secondary antibody was diluted at 1:300 room temperature. 30min(Assay Biotech SA0403). DAPI(blue) 10min.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HEK293T cells transfected with Luciferase . 1.Luciferase Mouse Monoclonal Antibody(6B8)(green) was diluted at 1:200(4 degree C.overnight). 2. Dylight 488 labled Secondary antibody was diluted at 1:300 room temperature. 30min(Assay Biotech SA0403). DAPI(blue) 10min.)
WB (Western Blot)
(Western Blot analysis of over-expressed Luciferase protein using antibody diluted at 1:1000)
WB (Western Blot)
(Western Blot analysis of over-expressed Luciferase protein using antibody diluted at 1:1000)
Related Product Information for anti-Luciferase antibody
Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Product Categories/Family for anti-Luciferase antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Luciferase luciferase (Catalog #AAA320515) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Luciferase Mouse Monoclonal Antibody (6B8) reacts with Firefly and may cross-react with other species as described in the data sheet. AAA Biotech's Luciferase can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the Luciferase luciferase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Luciferase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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