Rat Ly-6B.2 ALLOANTIGEN Monoclonal Antibody | anti-Ly-6B.2 antibody
RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
Ly-6B.2 ALLOANTIGEN, Antibody; RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:FITC; anti-Ly-6B.2 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7/4
Specificity
Recognizes the Ly-6B.2 antigen. Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-B6.2 to ~15 kDa (Rosas et al.2010).
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Applicable Applications for anti-Ly-6B.2 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunogen
Cultured bone marrow cells
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Fusion Partners
Spleen cells from AO rats were fused with cells from the Y3 Ag1.2.3 rat myeloma cell line.
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Max Ex/Em
Fluorophore: FITC
Excitation Max (nm): 490
Emission Max (nm): 525
Excitation Max (nm): 490
Emission Max (nm): 525
Preparation and Storage
Store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Similar Products
Product Notes
The Ly-6B.2 (Catalog #AAA12191) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ly-6B.2 ALLOANTIGEN can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the Ly-6B.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.