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product-image-AAA213670_WB8.jpg WB (Western Blot) (Detection of mouse LYRIC/AEG1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, ND7/23, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

LYRIC/AEG1 recombinant antibody

Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody [BLR258L]

Gene Names
MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
LYRIC/AEG1, Recombinant Antibody; Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody [BLR258L]; astrocyte elevated gene-1 protein; protein LYRIC; metastasis adhesion protein; Metadherin; lysine-rich CEACAM1 co-isolated protein; LYRIC; AEG-1; AEG1; 3D3/LYRIC; 3D3; astrocyte elevated gene 1; LYRIC/3D3; Protein LYRIC; metadherin; LYRIC/AEG1 recombinant antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR258L
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
250ug/ml (varies by lot)
Applicable Applications for LYRIC/AEG1 recombinant antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 475 and 525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse LYRIC/AEG1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, ND7/23, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

product-image-AAA213670_WB8.jpg WB (Western Blot) (Detection of mouse LYRIC/AEG1 by western blot. Samples: Whole cell lysate (25 ug) from NIH 3T3, ND7/23, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human LYRIC/AEG1 by western blot. Samples: Whole cell lysate (10 ug) from RKO, LNCaP, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

product-image-AAA213670_WB10.jpg WB (Western Blot) (Detection of human LYRIC/AEG1 by western blot. Samples: Whole cell lysate (10 ug) from RKO, LNCaP, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

ICC (Immunocytochemistry)

(Detection of mouse LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used for IP at 20 ul/mg lysate. LYRIC/AEG1 was also immunoprecipitated by a second antibody against a different epitope of LYRIC/AEG1 (BL-2259B-2C7). For blotting immunoprecipitated LYRIC/AEG1, AAA213670 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

product-image-AAA213670_ICC11.jpg ICC (Immunocytochemistry) (Detection of mouse LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670 lot 1) used for IP at 20 ul/mg lysate. LYRIC/AEG1 was also immunoprecipitated by a second antibody against a different epitope of LYRIC/AEG1 (BL-2259B-2C7). For blotting immunoprecipitated LYRIC/AEG1, AAA213670 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

FCM/FACS (Flow Cytometry)

(Detection of human LYRIC/AEG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

product-image-AAA213670_FCM13.jpg FCM/FACS (Flow Cytometry) (Detection of human LYRIC/AEG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM/FACS (Flow Cytometry)

(Detection of mouse LYRIC/AEG1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

product-image-AAA213670_FCM15.jpg FCM/FACS (Flow Cytometry) (Detection of mouse LYRIC/AEG1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (AAA213670) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
Related Product Information for LYRIC/AEG1 recombinant antibody
Astrocyte elevated gene-1 protein (AEG1) is also known as metadherin. AEG1 AEG1 promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. It also promotes lung metastasis and has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. AEG1 induces chemoresistance.
Product Categories/Family for LYRIC/AEG1 recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,837 Da
NCBI Official Full Name
protein LYRIC
NCBI Official Synonym Full Names
metadherin
NCBI Official Symbol
MTDH
NCBI Official Synonym Symbols
3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3
NCBI Protein Information
protein LYRIC
UniProt Protein Name
Protein LYRIC
UniProt Gene Name
MTDH
UniProt Synonym Gene Names
AEG1; LYRIC; AEG-1
UniProt Entry Name
LYRIC_HUMAN

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Product Notes

The LYRIC/AEG1 mtdh (Catalog #AAA213670) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody [BLR258L] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LYRIC/AEG1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LYRIC/AEG1 mtdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LYRIC/AEG1, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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