Application Data
(Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages were stained with anti-Mac-3 (clone: M3/84) (filled histogram) or rat IgG1 isotype control (open histogram))
Rat anti-Mouse Mac-3 Monoclonal Antibody
Anti-Mouse Mac-3, Biotin (Clone M3/84) (rat IgG1)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Synonyms
Mac-3, Antibody; Anti-Mouse Mac-3, Biotin (Clone M3/84) (rat IgG1); Mac-3, Biotin (Clone M3/84) (rat IgG1); Purified Anti-Mouse MAC-3 Monoclonal Antibody; anti-Mac-3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG 1K
Clone Number
M3/84
Specificity
Mac-3 (Mouse MAC-3)
Form/Format
Biotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3.
Concentration
0.1 mg/ml (varies by lot)
Applicable Applications for anti-Mac-3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
SPECIFICATIONS
CLONE: M3/84
IMMUNIZATION
Immunogen: Immunoadsobent purified mouse macrophage glycoprotein fraction.
Donor: Rat spleen
SPECIFITY: Mouse MAC-3
IMMUNIZATION
Immunogen: Immunoadsobent purified mouse macrophage glycoprotein fraction.
Donor: Rat spleen
SPECIFITY: Mouse MAC-3
TEST RESULTS
TISSUE DISTRIBUTION BY FLOW CYTOMETRY ANALYSIS:
Mouse Strain: C57BL/6
Cell Concentration: 1 *106 cells per test
Antibody Concentration Used: 1.0 ug/106
Cell Source: Thioglycollate-elicited peritoneal exudates
Percentage of cells stained above control: 69.4%
Mouse Strain: C57BL/6
Cell Concentration: 1 *106 cells per test
Antibody Concentration Used: 1.0 ug/106
Cell Source: Thioglycollate-elicited peritoneal exudates
Percentage of cells stained above control: 69.4%
Preparation and Storage
Store at 4°C. For long term storage (Purified, Biotin FITC, No Azide) aliquot and freeze unused portion at -20°C in volumes appropiate for single usage. Avoid freeze/thaw cycles.
Application Data
(Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages were stained with anti-Mac-3 (clone: M3/84) (filled histogram) or rat IgG1 isotype control (open histogram))
Application Data
(Thioglycolate-elicited C57BL/6 mouse peritoneal macrophages were stained with anti-Mac-3 (clone: M3/84) (filled histogram) or rat IgG1 isotype control (open histogram))
Application Data
Application Data
Related Product Information for anti-Mac-3 antibody
anti-mouse MAC-3 monoclonal antibody recognizes the MAC-3 antigen which is found on macrophages and some nonlymphoid tissues, but not on lymphocytes. It is a glycoprotein showing a broad band in SDS-PAGE, and is synthesized by macrophages (average molecular weight 92-110,000 daltons, depending on the origin of macrophages). It reacts with peritoneal exudate macrophages where the exudate is provoked by thioglycollate, protease peptone, L. monocytogenes, lipopolysaccharide and concanavalin A. It does not react with thymocytes, spleen, lymph node or bone marrow cells in immunofluorescent flow cytometry. MAC-3 is a general marker for macrophages and can be used to distinguish these cells from lymphocytes. Its Mr 110,000 and cell distribution differentiate MAC-3 from MAC-2 and MAC-1 antigens.
Similar Products
Product Notes
The Mac-3 (Catalog #AAA74210) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse Mac-3, Biotin (Clone M3/84) (rat IgG1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mac-3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the Mac-3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mac-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.