IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (SPM281))
Mouse Macrophage L1 Protein Monoclonal Antibody | anti-S100A8 antibody
Macrophage L1 Protein Mouse Monoclonal Antibody
Gene Names
S100A8; P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
Reactivity
Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea Pig, Rat, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
Macrophage L1 Protein, Antibody; Macrophage L1 Protein Mouse Monoclonal Antibody; 60B8AG; Calgranulin A (CAGA); Calgranulin B (CAGB); Calprotectin L1L subunit; Chemotactic cytokine CP-10; Cystic fibrosis antigen (CFAG); Leukocyte L1 complex light chain; L1Ag; Macrophage L1 protein Calprotectin; Migration inhibitory factor related protein 8; Myeloid-related protein 8 (MRP-8 or P8); Myeloid-related protein 14 (MRP-14 or P14); Neutrophil cytosolic 7kDa protein; NIF; p8; Migration inhibitory factor related protein 14; S100 calcium binding protein A8 (S100A8); S100 calcium binding protein A9 (S100A9); S100A8/S100A9 complex; MA387; Pro-inflammatory S100 cytokine; Urinary stone protein band A; anti-S100A8 antibody
Host
Mouse
Reactivity
Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea Pig, Rat, Mouse
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM281
Specificity
Recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12kDa alpha chain and a 14kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-S100A8 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Cellular Localization
Cytoplasmic
Immunogen
Affinity purified monocyte membrane preparation
Hu-Chromosome Location
1q21
Positive Control
Tonsil, lymph node, or spleen
Preparation and Storage
Antibody with azide - store at 2-8°C
Antibody without azide - store at -20°C to -80°C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20°C to -80°C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (SPM281))
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Macrophage L1 Protein Monoclonal Antibody (SPM281))
References
Flavell DJ; Jones DB; Wright DH. Identification of tissue histiocytes on paraffin sections by a new monoclonal antibody. Journal of Histochemistry and Cytochemistry, 1987, 35(11):1217-26.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12-14kDa (doublet)
NCBI Official Full Name
protein S100-A8 isoform b
NCBI Official Synonym Full Names
S100 calcium binding protein A8
NCBI Official Symbol
S100A8
NCBI Official Synonym Symbols
P8; MIF; NIF; CAGA; CFAG; CGLA; L1Ag; MRP8; CP-10; MA387; 60B8AG
NCBI Protein Information
protein S100-A8
UniProt Protein Name
Protein S100-A8
UniProt Gene Name
S100A8
UniProt Synonym Gene Names
CAGA; CFAG; MRP8; CFAG; MRP-8; p8
UniProt Entry Name
S10A8_HUMAN
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Product Notes
The S100A8 s100a8 (Catalog #AAA62807) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Macrophage L1 Protein Mouse Monoclonal Antibody reacts with Human, Baboon, Monkey, Cow, Pig, Goat, Horse, Cat, Dog, Rabbit, Guinea Pig, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Macrophage L1 Protein can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the S100A8 s100a8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage L1 Protein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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