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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Mad2L2 Monoclonal Antibody | anti-Mad2L2 antibody
Mad2L2 Antibody
Gene Names
MAD2L2; REV7; MAD2B; POLZ2
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Mad2L2, Antibody; Mad2L2 Antibody; Homolog of REV7 S cerevisiae antibody; hREV7 antibody; MAD2 (mitotic arrest deficient yeast; homolog) like 2 antibody; MAD2 homolog antibody; MAD2 like 2 antibody; MAD2 mitotic arrest deficient like 2 antibody; MAD2-like protein 2 antibody; MAD2B antibody; Mad2l2 antibody; MD2L2_HUMAN antibody; Mitotic Arrest Deficient 2 L2 antibody; Mitotic arrest deficient 2-like protein 2 antibody; Mitotic arrest deficient homolog like 2 antibody; Mitotic arrest deficient like 2 (yeast) antibody; Mitotic arrest deficient yeast homolog antibody; Mitotic spindle assembly checkpoint protein MAD2B antibody; Polymerase (DNA directed) zeta 2 accessory subunit antibody; POLZ2 antibody; REV 7 antibody; REV7 antibody; REV7 homolog antibody; Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody; anti-Mad2L2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU99-23
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-Mad2L2 antibody
IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.)
Related Product Information for anti-Mad2L2 antibody
Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.
Product Categories/Family for anti-Mad2L2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,334 Da
NCBI Official Full Name
mitotic spindle assembly checkpoint protein MAD2B
NCBI Official Synonym Full Names
MAD2 mitotic arrest deficient-like 2 (yeast)
NCBI Official Symbol
MAD2L2
NCBI Official Synonym Symbols
REV7; MAD2B; POLZ2
NCBI Protein Information
mitotic spindle assembly checkpoint protein MAD2B; hREV7; REV7 homolog; MAD2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic arrest deficient 2-like protein 2; polymerase (DNA-directed), zeta 2, accessory subunit; MAD2 (mitotic arrest def
UniProt Protein Name
Mitotic spindle assembly checkpoint protein MAD2B
UniProt Gene Name
MAD2L2
UniProt Synonym Gene Names
MAD2B; REV7; MAD2-like protein 2; hREV7
UniProt Entry Name
MD2L2_HUMAN
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Product Notes
The Mad2L2 mad2l2 (Catalog #AAA312574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mad2L2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mad2L2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Mad2L2 mad2l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mad2L2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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