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WB (Western Blot)
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WB (Western Blot) (Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 ug) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

MAFA recombinant antibody

Rabbit anti-MafA Recombinant Monoclonal Antibody [BLR067G]

Gene Names
Mafa; RIPE3b1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Purified
Synonyms
MafA; Monoclonal Recombinant Antibody; Rabbit anti-MafA Recombinant Monoclonal Antibody [BLR067G]; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; transcription factor RIPE3b1; transcription factor MafA; RIPE3b1 factor; RIPE3b1; pancreatic beta-cell-specific transcriptional activator; INSDM; hMafA; Transcription factor MafA; MAF bZIP transcription factor A; MAFA recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR067G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for MAFA recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF)
Application Notes
WB: 1:1000
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining.
Immunogen
Between 125 and 175
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 ug) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 ug) from Beta-TC-6, AlphaTC1 Clone 9, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot)

(Detection of human MafA by western blot. Samples: Whole cell lysate (10 ug) from HEK293T transfected with myc tagged Human MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag .)

WB (Western Blot) (Detection of human MafA by western blot. Samples: Whole cell lysate (10 ug) from HEK293T transfected with myc tagged Human MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag .)

WB (Western Blot)

(Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 ug) from HEK293T transfected with myc tagged mouse MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag .)

WB (Western Blot) (Detection of mouse MafA by western blot. Samples: Whole cell lysate (10 ug) from HEK293T transfected with myc tagged mouse MafA, Human MafB, and Human cMaf prepared using NETN lysis buffer. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit recombinant monoclonal antibody to Myc-tag .)

IHC (Immunohistochemistry)

(Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human MafA (red) by immunohistochemistry. Sample: FFPE section of human pancreatic carcinoma. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry)

(Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouse pancreas (JB fixation). Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouse pancreas (JB fixation). Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of human MafA by immunohistochemistry. Sample: FFPE section of human pancreatic tumor. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human MafA by immunohistochemistry. Sample: FFPE section of human pancreatic tumor. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouseBeta-TC-6 cells. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse MafA by immunohistochemistry. Sample: FFPE section of mouseBeta-TC-6 cells. Antibody: Rabbit anti-MafA recombinant monoclonal antibody (AAA23816 lot 4). Secondary: HRP-conjugated goat anti-rabbit IgG .)
Related Product Information for MAFA recombinant antibody
MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells.
Product Categories/Family for MAFA recombinant antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,576 Da
NCBI Official Full Name
transcription factor MafA
NCBI Official Synonym Full Names
v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
NCBI Official Symbol
Mafa
NCBI Official Synonym Symbols
RIPE3b1
NCBI Protein Information
transcription factor MafA; Mafa homolog; pancreatic beta-cell-specific transcriptional activator; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
UniProt Protein Name
Transcription factor MafA
UniProt Gene Name
MAFA
UniProt Entry Name
MAFA_HUMAN

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Product Notes

The MAFA mafa (Catalog #AAA23816) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MafA Recombinant Monoclonal Antibody [BLR067G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MafA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF). WB: 1:1000 IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Zinc-fixative (JB fix) is recommended to enhance staining. Researchers should empirically determine the suitability of the MAFA mafa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MafA, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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