WB (Western Blot)
(Detection of MafA, MafB and cMaf by western blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. AAA211052), anti-MafB Antibody (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.)
Rabbit anti-Mouse MafA Polyclonal Antibody | anti-MafA antibody
Rabbit anti-MafA Antibody, Affinity Purified
Gene Names
Mafa; RIPE3b1
Reactivity
Mouse
Applications
Western Blot
Purity
Antigen Affinity Purified
Synonyms
MafA, Antibody; Rabbit anti-MafA Antibody, Affinity Purified; Mafa homolog; pancreatic beta-cell-specific transcriptional activator; RIPE3b1; transcription factor MafA; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A; Transcription factor MafA; v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian); anti-MafA antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MafA antibody
WB (Western Blot)
Predicted Reactivity
Human
Immunogen
Between 309 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of MafA, MafB and cMaf by western blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. AAA211052), anti-MafB Antibody (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.)
WB (Western Blot)
(Detection of MafA, MafB and cMaf by western blot. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. AAA211052), anti-MafB Antibody (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No. Each antibody was used at 0.5 ug/ml. Detection: Chemiluminescence with a 5 second exposure.)
IP (Immunoprecipitation)
(Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody BL1069 (Cat. No. AAA211052).)
IP (Immunoprecipitation)
(Binding of MafA to the enhancer region of the endogenous insulin gene. Samples: Immunoprecipitated cross-linked DNA from betaTC-3 cells was analyzed by PCR. As controls, reactions were run with no DNA, with input chromatin, with DNA obtained after precipitation with rabbit IgG or without antibody (lanes 1 through 5, respectively). Antibody: Affinity purified rabbit anti-MafA antibody BL1069 (Cat. No. AAA211052).)
EMSA
(Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. AAA211052), anti-MafB Antibody BL658 (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No.)
EMSA
(Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069 (Cat. No. AAA211052), anti-MafB Antibody BL658 (Cat. No. A300-612A) or anti-c-Maf antibody BL662 (Cat. No.)
Related Product Information for anti-MafA antibody
MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells.
Product Categories/Family for anti-MafA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,576 Da
NCBI Official Full Name
transcription factor MafA
NCBI Official Synonym Full Names
v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
NCBI Official Symbol
Mafa
NCBI Official Synonym Symbols
RIPE3b1
NCBI Protein Information
transcription factor MafA; Mafa homolog; pancreatic beta-cell-specific transcriptional activator; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A
UniProt Protein Name
Transcription factor MafA
UniProt Gene Name
Mafa
UniProt Entry Name
MAFA_MOUSE
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Product Notes
The MafA mafa (Catalog #AAA211052) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MafA Antibody, Affinity Purified reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MafA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MafA mafa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MafA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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