WB (Western Blot)
(Detection of mouse MafB by western blot. Samples: Whole cell lysate (50 ug) from Beta-TC-6 and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panels: Rabbit anti-MafA recombinant monoclonal antibody and rabbit anti-Actin recombinant monoclonal antibody (AAA213545).)
MAFB recombinant antibody
Rabbit anti-MafB Recombinant Monoclonal Antibody [BLR046F]
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse MafB by western blot. Samples: Whole cell lysate (50 ug) from Beta-TC-6 and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panels: Rabbit anti-MafA recombinant monoclonal antibody and rabbit anti-Actin recombinant monoclonal antibody (AAA213545).)
WB (Western Blot)
(Detection of mouse MafB by western blot. Samples: Whole cell lysate (50 ug) from Beta-TC-6 and AlphaTC1 Clone 9 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panels: Rabbit anti-MafA recombinant monoclonal antibody and rabbit anti-Actin recombinant monoclonal antibody (AAA213545).)
WB (Western Blot)
(Detection of human MafB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, SK-N-MC, RPMI-8226, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin antibody .)
WB (Western Blot)
(Detection of human MafB by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, SK-N-MC, RPMI-8226, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin antibody .)
IP (Immunoprecipitation)
(Detection of human MafB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-MC cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used for IP at 6 ul/mg lysate. MafB was also immunoprecipitated by a previous lot of this antibody (AAA213545 lot 2). For blotting immunoprecipitated MafB, AAA213545 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MafB by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from SK-N-MC cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3) used for IP at 6 ul/mg lysate. MafB was also immunoprecipitated by a previous lot of this antibody (AAA213545 lot 2). For blotting immunoprecipitated MafB, AAA213545 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohiostchemistry)
(Detection of human MAFB in FFPE pancreatic tumor by immunohistochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human MAFB in FFPE pancreatic tumor by immunohistochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MAFB in FFPE SK-N-MC cells by immunocytochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MAFB in FFPE SK-N-MC cells by immunocytochemistry. Antibody: Rabbit anti-MafB recombinant monoclonal antibody (AAA213545 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
NCBI and Uniprot Product Information
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Product Notes
The MAFB mafb (Catalog #AAA213545) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MafB Recombinant Monoclonal Antibody [BLR046F] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MafB can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MAFB mafb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MafB, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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