Mouse anti-Human MBD3 Monoclonal Antibody | anti-MBD3 antibody

MBD3 Mouse Monoclonal Antibody [Clone PCRP-MBD3-1C4]

Cat. Num. AAA215840

• Reactivity: Human
• Applications: Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: MBD3, Antibody; MBD3 Mouse Monoclonal Antibody [Clone PCRP-MBD3-1C4]; Methyl CpG binding protein MBD3; Methyl-CpG-binding domain protein 3; Methyl-CpG-binding protein MBD3; anti-MBD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-MBD3-1C4
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-MBD3 antibody: IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Immunogen: Recombinant full-length human MBD3 protein
• Cellular Localization: Nucleus
• Positive Control: HeLa or K562 cells.
• Hu-chromosome Location: 19p13.3
• Unigene: 178728
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

FCM/FACS (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. MBD3 Mouse Monoclonal Antibody (PCRP-MBD3-1C4) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).)

Related Product Information for anti-MBD3 antibody

Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5' cytosine followed by guanosine (CpG), and it requires the enzymatic activity of DNA methyltransferase, which results in transcriptional repression of the methylated gene. Several proteins have been identified that associate with the methyl-CpG sites; they include methyl-CpG binding protein 1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. MBD1 binds in a context specific manner to methyl-CpG rich domains and, in turn, mediates the transcriptional inhibition that is commonly observed with DNA methylation. Similarly, MBD2 inhibits transcription of methylated genes by associating with histone deacetylase (HDAC1) within the MeCP1 repressor complex. In addition, MBD4, which is also designated MED1, associates with the mismatch repair protein MLH1 and preferentially binds to methylated cytosine residues in mismatched base pairs. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases.

NCBI and Uniprot Product Information

• NCBI GI #: 4505119
• NCBI GeneID: 53615
• NCBI Accession #: NP_003917.1
• NCBI GenBank Nucleotide #: NM_003926.5
• UniProt Accession #: O95983; Q6PIL9; Q6PJZ9; Q86XF4; A8K4B7; D6W5Z2
• Molecular Weight: 32,844 Da
• NCBI Official Full Name: methyl-CpG-binding domain protein 3
• NCBI Official Synonym Full Names: methyl-CpG binding domain protein 3
• NCBI Official Symbol: MBD3
• NCBI Protein Information: methyl-CpG-binding domain protein 3
• UniProt Protein Name: Methyl-CpG-binding domain protein 3
• UniProt Gene Name: MBD3
• UniProt Entry Name: MBD3_HUMAN