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product-image-AAA19377_FCM6.png FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-MCM5 antibody (AAA19377).Overlay histogram showing A549 cells stained with AAA19377 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM5 Antibody (AAA19377, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Mouse MCM5 Monoclonal Antibody | anti-MCM5 antibody

Anti-MCM5 Antibody (monoclonal, 4G10)

Gene Names
MCM5; CDC46; P1-CDC46
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified.
Synonyms
MCM5, Antibody; Anti-MCM5 Antibody (monoclonal, 4G10); MCM5; CDC46; DNA replication licensing factor MCM5; EC 3. 6. 4. 12; CDC46 homolog; P1-CDC46; minichromosome maintenance complex component 5; anti-MCM5 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
4G10
Specificity
Mouse IgG monoclonal antibody for MCCM5 detection.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Applicable Applications for anti-MCM5 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM)
Application Notes
WB: 0.25-0.5ug/ml|Human, Mouse, Rat|
IHC-P: 2-5ug/ml|Human|
ICC/IF: 5ug/ml|Human|
FC/FACS/FCM: 1-3ug/1x106 cells|Human|
Immunogen
E Coli-derived human MCM5 recombinant protein (Position: K206-H723).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Recommended Detection Systems
Recommended Detection Systems
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

FCM (Flow Cytometry)

(Figure 6. Flow Cytometry analysis of A549 cells using anti-MCM5 antibody (AAA19377).Overlay histogram showing A549 cells stained with AAA19377 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM5 Antibody (AAA19377, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

product-image-AAA19377_FCM6.png FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-MCM5 antibody (AAA19377).Overlay histogram showing A549 cells stained with AAA19377 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM5 Antibody (AAA19377, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence)

(Figure 5. IF analysis of MCM5 using anti- MCM5 antibody (AAA19377).MCM5 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- MCM5 Antibody (AAA19377) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

product-image-AAA19377_IF5.jpg IF (Immunofluorescence) (Figure 5. IF analysis of MCM5 using anti- MCM5 antibody (AAA19377).MCM5 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- MCM5 Antibody (AAA19377) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry)

(Figure 4. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

product-image-AAA19377_IHC4.jpg IHC (Immunohistochemistry) (Figure 4. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 3. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

product-image-AAA19377_IHC3.jpg IHC (Immunohistochemistry) (Figure 3. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry)

(Figure 2. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

product-image-AAA19377_IHC2.jpg IHC (Immunohistochemistry) (Figure 2. IHC analysis of MCM5 using anti-MCM5 antibody (AAA19377).MCM5 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-MCM5 Antibody (AAA19377) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot)

(Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (AAA19377).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: rat thymus tissue lysatesLane 3: rat RH-35 whole cell lysatesLane 4: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MCM5 antigen affinity purified monoclonal antibody (Catalog # AAA19377) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for MCM5 at approximately 97KD. The expected band size for MCM5 is at 97KD.)

product-image-AAA19377_WB.jpg WB (Western Blot) (Figure 1. Western blot analysis of MCM5 using anti-MCM5 antibody (AAA19377).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: rat thymus tissue lysatesLane 3: rat RH-35 whole cell lysatesLane 4: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- MCM5 antigen affinity purified monoclonal antibody (Catalog # AAA19377) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for MCM5 at approximately 97KD. The expected band size for MCM5 is at 97KD.)
Related Product Information for anti-MCM5 antibody
DNA replication licensing factor MCM5 is a protein that in humans is encoded by the MCM5 gene. It is mapped to 22q12. 3. The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
References
1. Seroussi, E., Kedra, D., Kost-Alimova, M., Sandberg-Nordqvist, A. -C., Fransson, I., Jacobs, J. F. M., Fu, Y., Pan, H. -Q., Roe, B. A., Imreh, S., Dumanski, J. P. TOM1 genes map to human chromosome 22q13. 1 and mouse chromosome 8C1 and encode proteins similar to the endosomal proteins HGS and STAM. Genomics 57: 380-388, 1999.
2. Tsuruga, H., Yabuta, N., Hashizume, K., Ikeda, M., Endo, Y., Nojima, H. Expression, nuclear localization and interactions of human MCM/P1 proteins. Biochem. Biophys. Res. Commun. 236: 118-125, 1997.
3. Vetro, A., Savasta, S., Russo Raucci, A., Cerqua, C., Sartori, G., Limongelli, I., Forlino, A., Maruelli, S., Perucca, P., Vergani, D., Mazzini, G., Mattevi, A., Stivala, L. A., Salviati, L., Zuffardi, O. MCM5: a new actor in the link between DNA replication and Meier-Gorlin syndrome. Europ. J. Hum. Genet. 25: 646-650, 2017.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
82,286 Da
NCBI Official Full Name
DNA replication licensing factor MCM5
NCBI Official Synonym Full Names
minichromosome maintenance complex component 5
NCBI Official Symbol
MCM5
NCBI Official Synonym Symbols
CDC46; P1-CDC46
NCBI Protein Information
DNA replication licensing factor MCM5; CDC46 homolog; minichromosome maintenance deficient 5 (cell division cycle 46); MCM5 minichromosome maintenance deficient 5, cell division cycle 46
UniProt Protein Name
DNA replication licensing factor MCM5
UniProt Gene Name
MCM5
UniProt Synonym Gene Names
CDC46
UniProt Entry Name
MCM5_HUMAN

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Product Notes

The MCM5 mcm5 (Catalog #AAA19377) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM5 Antibody (monoclonal, 4G10) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCM5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM). WB: 0.25-0.5ug/ml|Human, Mouse, Rat| IHC-P: 2-5ug/ml|Human| ICC/IF: 5ug/ml|Human| FC/FACS/FCM: 1-3ug/1x106 cells|Human|. Researchers should empirically determine the suitability of the MCM5 mcm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCM5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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