Application Data
(Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
Mouse MCP-1 Monoclonal Antibody | anti-MCP-1 antibody
MOUSE ANTI HUMAN MCP-1
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
MCP-1, Antibody; MOUSE ANTI HUMAN MCP-1; anti-MCP-1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2.2-4A4-1A11
Form/Format
Purified
Purified IgG - lyophilised
Purified IgG - lyophilised
Concentration
IgG concentration 1.0 mg/ml after reconstitution (varies by lot)
Sequence Length
99
Applicable Applications for anti-MCP-1 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Reconstitution
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. AAA Biotech recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Preparation
Histology Positive Control Tissue
Human Breast Invasive Ductal Carcinoma
Buffer Solution
Buffer Solution
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Application Data
(Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
Application Data
(Sandwich ELISA analysis of human MCP-1 using Mouse anti Human MCP-1 as a capture reagent and biotinylated Rabbit anti Human MCP-1 as a detection reagent with recombinant human MCP-1 as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are included undiluted)
Application Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded human breast ductal carcinoma stained with Mouse anti Human MCP-1 following antigen retrieval with sodium citrate buffer (pH6.0))
Related Product Information for anti-MCP-1 antibody
Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes human C-C motif chemokine 2, also known as Monocyte chemoattractant protein 1 (MCP-1), Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2 or CCL2, and does not cross react with MCP-2, MCP-3 or other structurally related chemokines. MCP-1 is a 99 amino acid, including a 23 aa signal peptide ~13kDa chemotactic protein, a chemoattractant for monocytes and basophils but not neutrophils or eosinophils (Weber et al.1996). MCP-1 is produced by a number of cell types, including monocytes, macrophages, microglia, fibroblasts and epithelial cells (Cushing et al. 1990). MCP-1 influences T-cell immunity polarizing responses towards the Th2 phenotype (Karpus and Kennedy 1997). Variations in the CCL2 gene affects susceptibility to Mycobacteriun tuberculosis infection (Flores-Villanueva et al. 2005). Mouse anti Human MCP-1 antibody, clone 2.2-4A4-1A11 recognizes both native and recombinant MCP-1 in conjunction with Rabbit anti Human MCP-1 polyclonal antibody in a sensitive sandwich ELISA assay.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,025 Da
NCBI Official Full Name
C-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 2
NCBI Official Symbol
CCL2
NCBI Official Synonym Symbols
HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
NCBI Protein Information
C-C motif chemokine 2; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein 1; monocyte secretory protein JE; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2
UniProt Protein Name
C-C motif chemokine 2
UniProt Gene Name
CCL2
UniProt Synonym Gene Names
MCP1; SCYA2; MCAF; MCP-1
UniProt Entry Name
CCL2_HUMAN
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Product Notes
The MCP-1 ccl2 (Catalog #AAA49386) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MCP-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MCP-1 ccl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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