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WB (Western Blot)
(Detection of human and mouse MEK2 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, A-549, Jurkat, RKO, U2OS, LNCaP, HeLa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
MEK2 recombinant antibody
Rabbit anti-MEK2 Recombinant Monoclonal Antibody [BLR079G]
Gene Names
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Purified
Synonyms
MEK2; Monoclonal Recombinant Antibody; Rabbit anti-MEK2 Recombinant Monoclonal Antibody [BLR079G]; MAPKK 2; MKK2; mitogen-activated protein kinase kinase 2; p45; PRKMK2; MAPKK2; MAP kinase kinase 2; ERK activator kinase 2; dual specificity mitogen-activated protein kinase kinase 2; CFC4; MEK 2; MAPK/ERK kinase 2; Dual specificity mitogen-activated protein kinase kinase 2; MEK2 recombinant antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
BLR079G
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for MEK2 recombinant antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
IP: 20 ul/mg lysate
IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MEK2 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, A-549, Jurkat, RKO, U2OS, LNCaP, HeLa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
WB (Western Blot)
(Detection of human and mouse MEK2 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, A-549, Jurkat, RKO, U2OS, LNCaP, HeLa, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)
IP (Immunoprecipitation)
(Detection of human MEK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1) used at 20 ul/mg lysate. For blotting immunoprecipitated MEK2, AAA23823 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MEK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1) used at 20 ul/mg lysate. For blotting immunoprecipitated MEK2, AAA23823 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human MEK2 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human MEK2 in FFPE prostate carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human MEK2 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry)
(Detection of human MEK2 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MEK2 in FFPE KG-1 cells by ICC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human MEK2 in FFPE KG-1 cells by ICC. Antibody: Rabbit anti-MEK2 recombinant monoclonal antibody (AAA23823 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry)
(Detection of human MEK2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK2 recombinant monoclonal (AAA23823 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human MEK2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-MEK2 recombinant monoclonal (AAA23823 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for MEK2 recombinant antibody
MEK2 (MAP2K2) is a dual specificity protein kinase that belongs to the MAP kinase kinase family. MEK2 is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3, and is involved in a multitude of cellular processes such as proliferation, differentiation, gene activation, and development [taken from NCBI Entrez Gene (Gene ID: 5605)].
Product Categories/Family for MEK2 recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
400
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 2
NCBI Official Symbol
MAP2K2
NCBI Official Synonym Symbols
CFC4; MEK2; MKK2; MAPKK2; PRKMK2
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 2; MAPK/ERK kinase 2; MAP kinase kinase 2; ERK activator kinase 2; mitogen-activated protein kinase kinase 2, p45
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 2
UniProt Gene Name
MAP2K2
UniProt Synonym Gene Names
MEK2; MKK2; PRKMK2; MAP kinase kinase 2; MAPKK 2; MEK 2
UniProt Entry Name
MP2K2_HUMAN
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Product Notes
The MEK2 map2k2 (Catalog #AAA23823) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MEK2 Recombinant Monoclonal Antibody [BLR079G] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MEK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the MEK2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK2, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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