Rabbit MEKK3 Monoclonal Antibody | anti-MEKK3 antibody

MEKK3 Antibody

Cat. Num. AAA30219

• Gene Names: MAP3K3; MEKK3; MAPKKK3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: MEKK3, Antibody; MEKK3 Antibody; M3K3_HUMAN antibody; MAP/ERK kinase kinase 3 antibody; MAP3K3 antibody; MAPK/ERK kinase kinase 3 antibody; MAPKKK3 antibody; MEK kinase 3 antibody; MEKK 3 antibody; MEKK3 antibody; Mitogen activated protein kinase kinase kinase 3 antibody; Mitogen-activated protein kinase kinase kinase 3 antibody; anti-MEKK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: SD0839
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-MEKK3 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; ICC: 1:100-1:500; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431)

Related Product Information for anti-MEKK3 antibody

MAP kinase kinase kinase (MEKK3 or MAP3K3) is a serine/threonine protein kinase that activates SAPK and ERK via phosphorylation and activation of their respective MAP kinase kinases, SEK and MEK1/2. MEKK3 also stimulates MEK5 via activation of ERK5/BMK1, which is at least partly regulated by a direct interaction between MEK5 and MEKK3 via p67phox-Bem1p (PB1) protein-protein interaction domains found in both proteins. MEKK3 modulates NF-kappaB activation in response to a variety of agonists including TNFalpha, LPS, IL-1 and LPA. Despite reports showing that phosphorylation of MEKK3 at Ser526 within the activation loop is necessary for kinase activation, at least one study suggests that dual phosphorylation at Thr516 and Ser520 is required for LPA-stimulated IKKbeta /NF-kappaB activation. Phosphorylation at Thr294 appears to negatively regulate MEKK3 by promoting 14-3-3beta binding and inhibition of the kinase activity.

Product Categories/Family for anti-MEKK3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 42794765
• NCBI GeneID: 4215
• NCBI Accession #: NP_002392.2
• NCBI GenBank Nucleotide #: NM_002401.3
• UniProt Accession #: Q99759; Q5BKZ6; Q8N3I9; B2RCW2; D3DU15
• Molecular Weight: 70,898 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: MAP3K3
• NCBI Official Synonym Symbols: MEKK3; MAPKKK3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3; MEKK 3; MEK kinase 3; MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: MAP3K3
• UniProt Synonym Gene Names: MAPKKK3; MEKK3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_HUMAN