FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Mouse anti-Human MEMO1 Monoclonal Antibody | anti-MEMO1 antibody
MEMO1
Gene Names
MEMO1; MEMO; C2orf4; CGI-27; NS5ATP7
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
MEMO1, Antibody; MEMO1; Protein MEMO1; C2orf4; CGI-27; MEMO; NS5ATP7; anti-MEMO1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT1E9
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
297
Applicable Applications for anti-MEMO1 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human MEMO1 (1-297aa) purified from E. coli
Conjugation
Unconjugated
Note
This product was produced from tissue culture supernatant.
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
FCM/FACS (Flow Cytometry)
(Flow cytometry analysis of MEMO1 in U87MG cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: U87MG cell lysate)
WB (Western Blot)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MEMO1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate Lane 2.: U87MG cell lysate)
Related Product Information for anti-MEMO1 antibody
MEMO1 (Mediator of ErbB2-driven cell motility 1) is a 297 amino acid protein. It is thought to relax extracellular chemotactic signals that are targeted at the microtubule cytoskeleton, thereby controlling cell migration. Additionally, MEMO1 is required for breast carcinoma migration, suggesting an important role in tumorigenesis. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity.
Product Categories/Family for anti-MEMO1 antibody
References
Marone R., et al. (2004) Nat Cell Biol 6(6):515-522; Meira M., et al. (2009) J Cell Sci 122:787-797; Zaoui K., et al. (2010) Proc Natl Acad Sci USA 107(43):18517-18522
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein MEMO1 isoform 1
NCBI Official Synonym Full Names
mediator of cell motility 1
NCBI Official Symbol
MEMO1
NCBI Official Synonym Symbols
MEMO; C2orf4; CGI-27; NS5ATP7
NCBI Protein Information
protein MEMO1
UniProt Protein Name
Protein MEMO1
UniProt Gene Name
MEMO1
UniProt Synonym Gene Names
C2orf4; MEMO; NS5ATP7; HCV NS5A-transactivated protein 7; Mediator of cell motility 1; Memo-1
UniProt Entry Name
MEMO1_HUMAN
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Product Notes
The MEMO1 memo1 (Catalog #AAA48338) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MEMO1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MEMO1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MEMO1 memo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEMO1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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