Application Data
(Measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.)
Rabbit MERS-CoV (NCoV/Novel coronavirus) Spike Monoclonal Antibody | anti-Spike antibody
MERS-CoV (NCoV/Novel coronavirus) Spike Antibody, Rabbit MAb
Reactivity
MERS-CoV
Applications
MN (Microneutralization)
Purity
Protein A
Synonyms
MERS-CoV (NCoV/Novel coronavirus) Spike, Antibody; MERS-CoV (NCoV/Novel coronavirus) Spike Antibody, Rabbit MAb; MERS-CoV Spike Antibody, Rabbit MAb; Anti-coronavirus s1 Antibody; Anti-coronavirus s2 Antibody; Anti-coronavirus spike Antibody; Anti-cov spike Antibody; Anti-ncov RBD Antibody; Anti-ncov s1 Antibody; Anti-ncov s2 Antibody; Anti-ncov spike Antibody; Anti-RBD Antibody; Anti-S Antibody; Anti-s1 Antibody; Anti-Spike RBD Antibody; anti-Spike antibody
Host
Rabbit
Reactivity
MERS-CoV
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
723
Specificity
The antibody reacts with MERS-CoV (NCoV/Novel coronavirus) full-length Spike protein (S protein).
Has cross-reactivity in ELISA with:
MERS-CoV Spike Protein (aa 1-1297, 40069-V08B)
MERS-CoV Spike Protein S1 (aa 1-725, 40069-V08B1)
MERS-CoV Spike Protein RBD (aa 367-606, 40071-V31B1)
No cross-reactivity in ELISA with:
MERS-CoV Spike Protein S2 (aa 726-1296, 40070-V08B)
Has cross-reactivity in ELISA with:
MERS-CoV Spike Protein (aa 1-1297, 40069-V08B)
MERS-CoV Spike Protein S1 (aa 1-725, 40069-V08B1)
MERS-CoV Spike Protein RBD (aa 367-606, 40071-V31B1)
No cross-reactivity in ELISA with:
MERS-CoV Spike Protein S2 (aa 726-1296, 40070-V08B)
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS, pH7.4
Applicable Applications for anti-Spike antibody
MN (Microneutralization)
Immunogen
Recombinant MERS-CoV (NCoV/Novel coronavirus) Spike Protein (ECD, aa 1-1297)
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant recombinant MERS-CoV (NCoV/Novel coronavirus) Spike Protein ( ; AFS88936.1 ; Met1-Trp1297).
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Avoid repeated freeze-thaw cycles.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
Avoid repeated freeze-thaw cycles.
Application Data
(Measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.)
Application Data
(Measured by its ability to inhibit infection of Caco-2 cells induced by MERS-CoV pseudovirus. The ED50 for this effect is 2.5-11 ng/mL.)
Related Product Information for anti-Spike antibody
Background: The spike protein is one of the four major structural proteins of the coronavirus. Coronavirus entry is mediated by the viral spike protein. The 180-kDa oligomeric spike protein of the murine coronavirus mouse hepatitis virus strain A59 is posttranslationally cleaved into an S1 receptor binding unit and an S2 membrane fusion unit. Both biochemical and functional data show that the coronavirus spike protein is a class I viral fusion protein. HCoV-EMC is a novel coronavirus isolated from a Saudi patient presenting with pneumonia and renal failure in June 2012. Genome sequencing showed that this virus belongs to the group C species of the genus betacoronavirus and phylogenetically related to the bat coronaviruses HKU4 and HKU5 previously found in lesser bamboo bat and Japanese Pipistrelle bat of Hong Kong respectively. HCoV-EMC has been identified as a highly lethal virus that could be passed from animals to humans very easily. The virus, like other coronaviridae, has the potential to infect bats and pigs in addition to humans. HCoV-EMC is related to the causative agent of SARS (severe acute respiratory syndrome), SARS coronavirus. and also may be even more infectious as it does not use the human receptor protein ACE2. The receptor used by HCov-EMC has not been identified. Symptoms of infection include kidney failure and severe pneumonia. The animal source has still not been identified but may circulate among populations of bats.
References
Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
NCBI and Uniprot Product Information
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Product Notes
The Spike (Catalog #AAA257037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MERS-CoV (NCoV/Novel coronavirus) Spike Antibody, Rabbit MAb reacts with MERS-CoV and may cross-react with other species as described in the data sheet. AAA Biotech's MERS-CoV (NCoV/Novel coronavirus) Spike can be used in a range of immunoassay formats including, but not limited to, MN (Microneutralization). Researchers should empirically determine the suitability of the Spike for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MERS-CoV (NCoV/Novel coronavirus) Spike, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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