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IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution)
Rabbit anti-Human MGEA5 Monoclonal Antibody | anti-MGEA5 antibody
MGEA5 Antibody
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Affinity-chromatography
Synonyms
MGEA5, Antibody; MGEA5 Antibody; Beta-hexosaminidase antibody; Beta-N-acetylglucosaminidase antibody; Beta-N-acetylhexosaminidase antibody; Bifunctional protein NCOAT antibody; HEXC antibody; Hyaluronidase in meningioma antibody; KIAA0679 antibody; MEA5 antibody; Meningioma expressed antigen 5 antibody; Meningioma-expressed antigen 5 antibody; MGEA 5 antibody; Mgea5 antibody; N-acetyl-beta-D-glucosaminidase antibody; N-acetyl-beta-glucosaminidase antibody; NCOAT antibody; Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody; OGA antibody; OGA_HUMAN antibody; Protein O-GlcNAcase antibody; anti-MGEA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JG40-05
Purity/Purification
Affinity-chromatography
Form/Format
Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05%bsa, 0.02% sodium azide and 50% glycerol
Applicable Applications for anti-MGEA5 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluoresence (IF)
Application Notes
WB 1:500-1:2000
IHC 1:100-1:200
IF 1:50-1:200
IP 1:20-1:50
IHC 1:100-1:200
IF 1:50-1:200
IP 1:20-1:50
Immunogen
A synthesized peptide derived from human MGEA5
Preparation and Storage
Store at +4°C for short term. Store at -20°C for long term. Avoid freeze/thaw cycle
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:50 dilution.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGEA5 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MGEA5 expression in JAR cell lysate.)
WB (Western Blot)
(Western blot analysis of MGEA5 expression in JAR cell lysate.)
WB (Western Blot)
(All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.)
WB (Western Blot)
(All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.)
WB (Western Blot)
(Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of MGEA5 on PC-3M and SH-SY-5Y cell lysates using anti-MGEA5 antibody at 1/500 dilution.)
Related Product Information for anti-MGEA5 antibody
Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
Product Categories/Family for anti-MGEA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 103kDa
SDS-PAGE MW: 130kDa
SDS-PAGE MW: 130kDa
NCBI Official Full Name
protein O-GlcNAcase isoform b
NCBI Official Synonym Full Names
meningioma expressed antigen 5 (hyaluronidase)
NCBI Official Symbol
MGEA5
NCBI Official Synonym Symbols
OGA; MEA5; NCOAT
NCBI Protein Information
protein O-GlcNAcase
UniProt Protein Name
Protein O-GlcNAcase
UniProt Gene Name
MGEA5
UniProt Synonym Gene Names
HEXC; KIAA0679; MEA5; NCOAT
Similar Products
Product Notes
The MGEA5 mgea5 (Catalog #AAA30518) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGEA5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluoresence (IF) . WB 1:500-1:2000 IHC 1:100-1:200 IF 1:50-1:200 IP 1:20-1:50 . Researchers should empirically determine the suitability of the MGEA5 mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGEA5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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