WB (Western Blot)
(Detection of human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody AAA212635 (lot AAA212635-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human MGEA5 Polyclonal Antibody | anti-MGEA5 antibody
Rabbit anti-MGEA5 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MGEA5, Antibody; Rabbit anti-MGEA5 Antibody, Affinity Purified; hyaluronidase in meningioma; MGEA5; OGA; nuclear cytoplasmic O-GlcNAcase and acetyltransferase; NCOAT; N-acetyl-beta-glucosaminidase; N-acetyl-beta-D-glucosaminidase; protein O-GlcNAcase; meningioma-expressed antigen 5; MEA5; bifunctional protein NCOAT; beta-N-acetylhexosaminidase; beta-N-acetylglucosaminidase; beta-hexosaminidase; meningioma expressed antigen 5 (hyaluronidase); Protein O-GlcNAcase; O-GlcNAcase; anti-MGEA5 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MGEA5 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 525 and 575
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody AAA212635 (lot AAA212635-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human MGEA5 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MGEA5 antibody AAA212635 (lot AAA212635-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human MGEA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MGEA5 antibody AAA212635 (lot AAA212635-2) used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212635-1) and rabbit anti-MGEA5 antibody BL15722. For blotting immunoprecipitated MGEA5, AAA212635 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human MGEA5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MGEA5 antibody AAA212635 (lot AAA212635-2) used for IP at 6 ug per reaction. MGEA5 was also immunoprecipitated by a previous lot of this antibody (lot AAA212635-1) and rabbit anti-MGEA5 antibody BL15722. For blotting immunoprecipitated MGEA5, AAA212635 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human MGEA5 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MGEA5 (Cat. No. AAA212635 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human MGEA5 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-MGEA5 (Cat. No. AAA212635 lot 2) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).)
Related Product Information for anti-MGEA5 antibody
Meningioma-expressed antigen 5 (MGEA5) cleaves GlcNAc but not GalNAc from glycopeptides. MGEA5 can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc.MGEA5 possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60502].
Product Categories/Family for anti-MGEA5 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MGEA5 mgea5 (Catalog #AAA212635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MGEA5 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGEA5 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MGEA5 mgea5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGEA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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