Rat anti-Mouse MHC Class II Monoclonal Antibody | anti-MHC antibody

Anti-Ms MHC Class II Biotin

Cat. Num. AAA129138

• Reactivity: Mouse
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Synonyms: MHC Class II, Antibody; Anti-Ms MHC Class II Biotin; anti-MHC antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2b
• Clone Number: M5/114
• Specificity: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
• Purity/Purification: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MHC antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Conjugation: Biotin
• Immunogen: Activated C57BL/6 mouse spleen cells
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-MHC antibody

MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

References

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