FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rat IgG isotype control (2 ug/Test,left) or MHC Class II (I-A/I-E) Rat mAb (AAA283070,2 ug/mL,right) , followed by PE goat anti-Rat pAb staining.)
Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody | anti-MHC antibody
MHC Class II (I-A/I-E) Rat mAb
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
MHC Class II (I-A/I-E), Antibody; MHC Class II (I-A/I-E) Rat mAb; Eb; Ia4; H2Eb; Ia-4; H-2Eb;IAb; Ia2; Ia-2; Abeta; H-2Ab; H2-Ab; Rmcs1; I-Abeta; Ia5; Ia-5; H-2Eb2; Iebeta2; A130038H09Rik; Ia1; H2Aa; Ia-1; H-2Aa; Aalpha; IAalpha; I-Aalpha; anti-MHC antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Applicable Applications for anti-MHC antibody
ELISA, FCM/FACS (Flow Cytometry)
Immunogen
Recombinant protein of mouse MHC Class II (I-A/I-E).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rat IgG isotype control (2 ug/Test,left) or MHC Class II (I-A/I-E) Rat mAb (AAA283070,2 ug/mL,right) , followed by PE goat anti-Rat pAb staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes were surface-stained with Rat IgG isotype control (2 ug/Test,left) or MHC Class II (I-A/I-E) Rat mAb (AAA283070,2 ug/mL,right) , followed by PE goat anti-Rat pAb staining.)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 Neuro-2a cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with MHC Class II (I-A/I-E) Rat mAb (AAA283070,2 ug/mL,orange line) or Rat IgG isotype control (2 ug/Test,blue line), followed by PE goat anti-Rat pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
FCM/FACS (Flow Cytometry)
(Flow cytometry: 1X10^6 Neuro-2a cells (negative control,left) and C57BL/6 mouse splenocytes (right) were surface-stained with MHC Class II (I-A/I-E) Rat mAb (AAA283070,2 ug/mL,orange line) or Rat IgG isotype control (2 ug/Test,blue line), followed by PE goat anti-Rat pAb staining. Non-fluorescently stained cells were used as blank control (red line).)
Related Product Information for anti-MHC antibody
Predicted to enable several functions, including MHC class II receptor activity; peptide antigen binding activity; and signaling receptor binding activity. Involved in response to interferon-gamma. Acts upstream of or within antigen processing and presentation of exogenous peptide antigen via MHC class II. Predicted to be located in external side of plasma membrane and immunological synapse. Predicted to be part of MHC class II protein complex. Predicted to be active in late endosome membrane and lysosomal membrane. Is expressed in lung. Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); bacterial infectious disease (multiple); eye disease (multiple); and sarcoidosis (multiple). Orthologous to human HLA-DRB1 (major histocompatibility complex, class II, DR beta 1) and HLA-DRB5 (major histocompatibility complex, class II, DR beta 5).
Product Categories/Family for anti-MHC antibody
NCBI and Uniprot Product Information
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Product Notes
The MHC h2-eb1 (Catalog #AAA283070) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The MHC Class II (I-A/I-E) Rat mAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MHC Class II (I-A/I-E) can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the MHC h2-eb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC Class II (I-A/I-E), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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