Application Data
(Fig.2: Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE, Agilent 2100 Bioanalyzer. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Mouse anti-Human MIC-1 Monoclonal Antibody | anti-GDF15 antibody
anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody
Gene Names
GDF15; PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein G
Synonyms
MIC-1, Antibody; anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody; GDF15; anti-GDF15 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
ME-6D10
Specificity
anti-human Macrophage Inhibitory Cytokine-1 (MIC-1)
Purity/Purification
Protein G
Form/Format
Purified
Sequence Length
308
Applicable Applications for anti-GDF15 antibody
ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry)
Buffer
phosphate buffered saline, pH 7.2
Immunogen
genetic immunisation with cDNA encoding aa30-194 (N-term) of MIC-1 proprotein
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Immunogen
cDNA
Preparation and Storage
Short term: 2-8°C; long term: -20°C (avoid repeated freezing and thawing)
Application Data
(Fig.2: Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE, Agilent 2100 Bioanalyzer. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.2: Capillary gel electrophoresis of purified ME-1C4 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: purified ME-1C4 antibody. Proteins were separated by CGE, Agilent 2100 Bioanalyzer. Internal control bands (240 kDa / 7 kDa / 4,5 kDa).)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using ME-6D10 AAA71479. BOSC23 cells were transiently transfected with an expression vector encoding either MIC-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ME-6D10 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 transfected cells.)
Application Data
(Fig.1: FACS analysis of BOSC23 cells using ME-6D10 AAA71479. BOSC23 cells were transiently transfected with an expression vector encoding either MIC-1 (red curve) or an irrelevant protein (control transfectant: black curve). Binding of ME-6D10 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with MIC-1 transfected cells.)
Related Product Information for anti-GDF15 antibody
Purified monoclonal mouse antibody.Background: Macrophage inhibitory cytokine-1 (MIC-1) is a member of the transforming growth factor-ß superfamily that regulates a wide variety of physiological processes involved in tissue differentiation and maintenance (1). MIC-1 is synthesized as a 62-kDa intracellular protein, which, after cleavage by a furin like protease, is secreted as a 25-kDa disulphide linked dimeric protein. Expression of MIC-1 results in inhibition of macrophage activation, regulated by the p53 pathway, in response to pro-inflammatory monokines (1,2). MIC-1 is involved in tumour pathogenesis and its measurement can be used as a clinical tool for the identification of a wide range of cancers. It could be a useful marker for aggressive prostate cancer while MIC-1 is upregulated in advanced and more aggressive prostatic tumours (3).
References
Hayes VM, Severi G, Southey MC, Padilla EJD, English DR, Hopper JL, Giles GG and Sutherland RL (2006). Macrophage Inhibitory Cytokine-1 H6D Polymorphism, Prostate Cancer Risk, and Survival. Cancer Epidemiology Biomarkers & Prevention 15, 1223-1225
Bootcov MR, Bauskin AR, Valenzuela SM, Moore AG, Bansal M, He XY, Zhang HP, Donnellan M, Mahler S, Pryor K, Walsh BJ, Nicholson RC, Fairlie WD, Por SB, Robbins JM and Breit SN (1997). MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily. Proc Natl Acad Sci U S A 14;94(21):11514-9
Bauskin AR, Brown DA, Kuffner T, Johnen H, Luo XW, Hunter M and Breit SN (2006). Role of Macrophage Inhibitory Cytokine-1 in Tumorigenesis and Diagnosis of Cancer. Cancer Research 66, 4983-4986
Bootcov MR, Bauskin AR, Valenzuela SM, Moore AG, Bansal M, He XY, Zhang HP, Donnellan M, Mahler S, Pryor K, Walsh BJ, Nicholson RC, Fairlie WD, Por SB, Robbins JM and Breit SN (1997). MIC-1, a novel macrophage inhibitory cytokine, is a divergent member of the TGF-beta superfamily. Proc Natl Acad Sci U S A 14;94(21):11514-9
Bauskin AR, Brown DA, Kuffner T, Johnen H, Luo XW, Hunter M and Breit SN (2006). Role of Macrophage Inhibitory Cytokine-1 in Tumorigenesis and Diagnosis of Cancer. Cancer Research 66, 4983-4986
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
growth/differentiation factor 15
NCBI Official Synonym Full Names
growth differentiation factor 15
NCBI Official Symbol
GDF15
NCBI Official Synonym Symbols
PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
NCBI Protein Information
growth/differentiation factor 15; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; prostate differentiation factor; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1
UniProt Gene Name
GDF15
UniProt Synonym Gene Names
MIC1; PDF; PLAB; PTGFB; GDF-15; MIC-1; NAG-1; NRG-1
UniProt Entry Name
GDF15_HUMAN
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Product Notes
The GDF15 gdf15 (Catalog #AAA71479) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human Macrophage Inhibitory Cytokine-1 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIC-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Competitive ELISA), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the GDF15 gdf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIC-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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