Mouse Microcystin LR Monoclonal Antibody
Microcystin LR monoclonal antibody (MC10E7)
Reactivity
Species independent
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G affinity purified
Synonyms
Microcystin LR, Antibody; Microcystin LR monoclonal antibody (MC10E7); anti-Microcystin LR antibody
Host
Mouse
Reactivity
Species independent
Clonality
Monoclonal
Isotype
IgG1
Clone Number
MC10E7
Specificity
Recognizes all 4-Arg microcystins
Purity/Purification
Protein G affinity purified
Form/Format
Lyophilized
Applicable Applications for anti-Microcystin LR antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Reconstitution
Reconstitute with 200ul PBS containing sodium azide
Immunogen
Microcystin-LR linked via N-methyl-dehydroalanine to cationized ovalbumin
Scientific Background
Microcystins are extremely toxic compounds produced by cyanobacteria (blue-green algae), belonging to species of Microcystis, Oscillatoria, Anabaena and Nostoc. The contamination of drinking water or water of recreational areas can cause severe health problems to exposed humans and animals. Microcystins possess a cyclic heptapeptide structure of the general composition cyclo(-D-Ala-L-X-D-erythro-β-methylisoAsp-L-Y-Adda-D-iso-Glu-N-methyldehydroAla), where Adda is the unusual C20 aa 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid and X and Y are variable L-aa.
Preparation and Storage
Storage at 4°C will decrease antibody activity and must be avoided.
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C (long term).
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C (long term).
Related Product Information for anti-Microcystin LR antibody
With a direct competitive enzyme-linked immunosorbent assay (ELISA) using antibody MC10E7, IC50 values for microcystin-LR of 0.06 ug/l have been obtained. The provisional guideline value proposed by the WHO is 1 µg/l for drinking water. The detection limit for microcystin-LR is 0.006 ug/l. All microcystin variants containing an arginine at position 4 show similar IC50 values and detection limits, whereas other microcystins, such as microcystin-LA are not recognized. The affinity constant for MC10E7 was determined to be at least 7x1010 l/mol.The antibody was tested for its robustness against interferences (humic acids, pH, salt content, surfactants or organic solvents) and was found to be very stable. Microcystin-LR spiked water samples in the concentration range between 0.01 and 0.1 ug/l were measured and a mean recovery of 99.9±16.4% was found. The antibody is well suited for the sensitive analysis of microcystins in drinking as well as surface water.
Product Categories/Family for anti-Microcystin LR antibody
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Product Notes
The Microcystin LR (Catalog #AAA77041) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Microcystin LR monoclonal antibody (MC10E7) reacts with Species independent and may cross-react with other species as described in the data sheet. AAA Biotech's Microcystin LR can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the Microcystin LR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Microcystin LR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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