WB (Western Blot)
(Western blot analysis of extracts from Raw264.7 cell lysates using MMP-2 mouse mAb (1:200 diluted). Predicted band size:64,72KDa. Observed band size:72KDa.)
Mouse anti-Human, Rat MMP-2 Monoclonal Antibody | anti-MMP-2 antibody
Anti-MMP-2 Antibody
Gene Names
MMP2; CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
Reactivity
Human, Rat
Applications
Immunofluorescence, Western Blot
Synonyms
MMP-2, Antibody; Anti-MMP-2 Antibody; 72 kDa gelatinase; 72kD type IV collagenase; CLG 4; CLG 4A; CLG4; CLG4A; Collagenase Type 4 alpha; Collagenase type IV A; Gelatinase A; Gelatinase alpha; Gelatinase neutrophil; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase; Matrix metalloproteinase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase); Matrix Metalloproteinase 2; Matrix metalloproteinase II; Matrix metalloproteinase-2; MMP 2; MMP II; MMP-2; MMP2; MMP2_HUMAN; MONA; Neutrophil gelatinase; PEX; TBE 1; TBE-1.; anti-MMP-2 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Form/Format
PBS (pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Sequence Length
610
Applicable Applications for anti-MMP-2 antibody
IF (Immunofluorescence), WB (Western Blot)
Cellular Location
Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes
Tissue Location
Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt
WB (Western Blot)
(Western blot analysis of extracts from Raw264.7 cell lysates using MMP-2 mouse mAb (1:200 diluted). Predicted band size:64,72KDa. Observed band size:72KDa.)
WB (Western Blot)
(Western blot analysis of extracts from Raw264.7 cell lysates using MMP-2 mouse mAb (1:200 diluted). Predicted band size:64,72KDa. Observed band size:72KDa.)
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20 degree C and using MMP-2 mouse mAb (dilution 1:50). DAPI was used to stain nucleus (blue).)
ICC (Immunocytochemistry)
(Immunofluorescent analysis of Hela cells fixed fixed by anhydrous methanol at -20 degree C and using MMP-2 mouse mAb (dilution 1:50). DAPI was used to stain nucleus (blue).)
WB (Western Blot)
(Western blot analysis of extracts from CHO-K1 (B) and CHO-K1 transfected by MMP-2 fragment (A) cell lysates using MMP-2 mouse mAb (1:2000 diluted). Predicted band size:30KDa. Observed band size:30KDa.)
WB (Western Blot)
(Western blot analysis of extracts from CHO-K1 (B) and CHO-K1 transfected by MMP-2 fragment (A) cell lysates using MMP-2 mouse mAb (1:2000 diluted). Predicted band size:30KDa. Observed band size:30KDa.)
Related Product Information for anti-MMP-2 antibody
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
72 KDa
NCBI Official Full Name
72 kDa type IV collagenase isoform 2
NCBI Official Synonym Full Names
matrix metallopeptidase 2
NCBI Official Symbol
MMP2
NCBI Official Synonym Symbols
CLG4; MONA; CLG4A; MMP-2; TBE-1; MMP-II
NCBI Protein Information
72 kDa type IV collagenase
UniProt Protein Name
72 kDa type IV collagenase
UniProt Gene Name
MMP2
UniProt Synonym Gene Names
CLG4A; MMP-2
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Product Notes
The MMP-2 mmp2 (Catalog #AAA290868) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-MMP-2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP-2 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MMP-2 mmp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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