Mouse anti Human IgG2 (Fab subclass specific) Secondary Antibody
Mouse anti Human IgG2 (Fab subclass specific)
Reactivity
Human IgG
Applications
ELISA, Immunofluorescence
Synonyms
Mouse anti Human IgG2 (Fab subclass specific), Secondary Antibody; Mouse anti Human IgG2 (Fab subclass specific); Mouse anti Human IgG2 (Fab subclass specific) secondary antibody
Host
Mouse
Reactivity
Human IgG
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NI 6014 (HP 6014)
Specificity
Monoclonal Mouse antibody to Human IgG2, subclass specific.
Form/Format
Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 1.0 mg/ml. No foreign proteins added.
Applicable Applications for Mouse anti Human IgG2 (Fab subclass specific) secondary antibody
ELISA, IF (Immunofluorescence)
Host Note
Highly purified monoclonal IgG2 isolated from pooled human serum
Reconstitution
Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
Reactivity Note
The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
Preparation and Storage
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4ºC, not refrozen, and preferably used the same day.
Related Product Information for Mouse anti Human IgG2 (Fab subclass specific) secondary antibody
The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Product Categories/Family for Mouse anti Human IgG2 (Fab subclass specific) secondary antibody
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Product Notes
The Mouse anti Human IgG2 (Fab subclass specific) (Catalog #AAA77529) is a Secondary Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Human IgG2 (Fab subclass specific) reacts with Human IgG and may cross-react with other species as described in the data sheet. AAA Biotech's Mouse anti Human IgG2 (Fab subclass specific) can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the Mouse anti Human IgG2 (Fab subclass specific) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mouse anti Human IgG2 (Fab subclass specific), Monoclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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