Mouse MPO Monoclonal Antibody | anti-MPO antibody

MPO, Mouse, mAb 8F4

Cat. Num. AAA77797

• Reactivity: Reactivity: Mouse. Cross reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Synonyms: MPO, Antibody; MPO, Mouse, mAb 8F4; anti-MPO antibody

• Host: Mouse
• Reactivity: Reactivity: Mouse. Cross reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: For 0.1 mg: 1 ml (100 µg/ml) 0.2 µm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For 0.5 and higher: PBS only (concentration< 0.5mg/ml); For unit size 0.5 mg or larger
• Sequence Length: 745

• Applicable Applications for anti-MPO antibody: IA (Immunoassay), FCM/FACS (Flow Cytometry), Activity Assay
• Immunogen: Purified mouse MPO from WEHI-3 cells
• Negative Control: Lymphocytes isolated from digested infarcts
• Positive Control: Neutrophils isolated from digested infarcts
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-MPO antibody

Product Description: The monoclonal antibody 8F4 recognizes mouse myeloperoxidase (MPO). MPO is a glycoprotein produced as a single precursor, which is subsequently cleaved into a alpha and beta chain. In human the biologically active MPO is a 150kDa tetramer composed of 2 glycosylated alfa chains of 59-64 kDa and 2 beta chains of 14 kDa.- MPO is- stored in azurophilic granules of polymorphonuclear leukocytes and is rapidly released into the phagosome and extracellular space during inflammatory conditions.The enzyme catalyzes the conversion of chloride and hydrogen peroxide to hypochlorite, a potent oxidant, which functions in host defense against microorganisms.
Involvement of MPO has been described in several human diseases, such as cardiovascular disease, airway inflammation, lung cancer, Alzheimer's disease and multiple sclerosis. A positive correlation between elevated MPO levels in serum and cardiovascular disease suggest an interesting role for MPO as an marker, making it possible to identify patients at risk for future cardiac events. Furthermore, there are some autoimmune diseases, in which MPO is targeted by antineutrophil cytoplasm antibodies. Studies with MPO-knockout mice have shown an- increased susceptibility to pneumonia following intratracheal infections. Moreover, MPO deficient- mice are more susceptible to experimental autoimmune encephalitis, a T cell-dependent neuronal disease, and have an increased expression of arteriosclerotic plaques compared to wild-type mice.
The anti-mouse MPO monoclonal antibody 8F4 recognizes natural MPO in biological solutions by ELISA,- in frozen tissue sections fixed with acetone and in flow cytometry using a- cell permeabilization method. The antibody 8F4 is cross-reactive- with rat MPO

References

1. Huugen, D et al; Aggravation of anti-myeloperoxidase antibody-induced glomerulonephritis by bacterial lipopolysaccharide: role of tumor necrosis factor-alpha. Am J Pathol 2005, 167: 47 2. Wang, D et al; C/EBPalpha directs monocytic commitment of primary myeloid progenitors. Blood 2006, 108:1223
3. Matthijsen, M et al; Myeloperoxidase is critically involved in the induction of organ damage after renal ischemia reperfusion. Am J Pathol 2007, 171: 1743
4. Leeuwen van, M et al; Accumulation of myeloperoxidase-positive neutrophils in atherosclerotic lesions in LDLR -/- mice. Arterioscler Thromb Vasc Biol 2008, 28: 84
5. Nahrendorf, M et al; An activatable MR imaging agent reports myeloperoxidase activity in healing infarcts and detects the anti-inflammatory effects of atorvastatin on ischemia-reperfusion injury non-invasively. Circulation 2008, 117: 1153

NCBI and Uniprot Product Information

• NCBI GI #: 4557759
• NCBI GeneID: 4353
• NCBI Accession #: NP_000241.1
• NCBI GenBank Nucleotide #: NM_000250.1
• Molecular Weight: 87,248 Da
• NCBI Official Full Name: myeloperoxidase
• NCBI Official Synonym Full Names: myeloperoxidase
• NCBI Official Symbol: MPO
• NCBI Protein Information: myeloperoxidase
• UniProt Protein Name: Myeloperoxidase
• UniProt Gene Name: MPO
• UniProt Synonym Gene Names: MPO
• UniProt Entry Name: PERM_HUMAN