IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279147 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
MRAS recombinant antibody
MRAS Recombinant Monoclonal Antibody
Gene Names
MRAS; M-RAs; RRAS3; R-RAS3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, ELISA
Purity
Affinity purified
Synonyms
MRAS, Recombinant Antibody; MRAS Recombinant Monoclonal Antibody; FLJ42964; M ras; M-ras; Mras; Muscle and microspikes Ras; Muscle RAS oncogene homolog; Muscle Ras oncogene homologue; Muscle Ras viral oncogene homolog; R ras3; R-ras3; Ras related protein MRas; Ras related protein RRas3; Ras-related protein M-Ras; Ras-related protein R-Ras3; RASM_HUMAN; Related Ras viral oncogene homolog 3; RRas3; XRas; MRAS recombinant antibody
Host
HEK293F Cell
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
26E12
Purity/Purification
Affinity purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for MRAS recombinant antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human MRAS protein
Conjugation
Non-conjugated
Research Area
Cancer; Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279147 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence)
(Immunofluorescence staining of Hela cell with AAA279147 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistochemistry)
(IHC image of AAA279147 diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohistochemistry)
(IHC image of AAA279147 diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,542 Da
NCBI Official Full Name
ras-related protein M-Ras isoform 1
NCBI Official Synonym Full Names
muscle RAS oncogene homolog
NCBI Official Symbol
MRAS
NCBI Official Synonym Symbols
M-RAs; RRAS3; R-RAS3
NCBI Protein Information
ras-related protein M-Ras; muscle and microspikes RAS; ras-related protein R-Ras3
UniProt Protein Name
Ras-related protein M-Ras
UniProt Gene Name
MRAS
UniProt Synonym Gene Names
RRAS3
UniProt Entry Name
RASM_HUMAN
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Product Notes
The MRAS mras (Catalog #AAA279147) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The MRAS Recombinant Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MRAS can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the MRAS mras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRAS, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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