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product-image-AAA23917_APP7.jpg Application Data (Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Mouse anti-Human MSH6 Monoclonal Antibody | anti-MSH6 antibody

MSH6 (DNA Mismatch Repair Protein)

Gene Names
MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
MSH6, Antibody; MSH6 (DNA Mismatch Repair Protein); DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC5; HSAP; MSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein; anti-MSH6 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
MSH6/3086
Specificity
The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
1360
Applicable Applications for anti-MSH6 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
ELISA: For coating, order Ab without BSA
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Positive Control
HCT116 or HeLa cell lysate (WB). Human colon carcinoma (IHC).
Immunogen
Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)
Cellular Localization
Nucleus.
Hu-chromosome Location
2p16.3
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Application Data

(Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

product-image-AAA23917_APP7.jpg Application Data (Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

WB (Western Blot)

(Western Blot Analysis of HCT116 cell lysate using MSH6 Mouse Monoclonal Antibody (MSH6/3086).)

product-image-AAA23917_WB6.jpg WB (Western Blot) (Western Blot Analysis of HCT116 cell lysate using MSH6 Mouse Monoclonal Antibody (MSH6/3086).)

SDS-PAGE

(SDS-PAGE Analysis Purified MSH6 Mouse Monoclonal Antibody (MSH6/3086). Confirmation of Purity and Integrity of Antibody.)

product-image-AAA23917_SDS_PAGE5.jpg SDS-PAGE (SDS-PAGE Analysis Purified MSH6 Mouse Monoclonal Antibody (MSH6/3086). Confirmation of Purity and Integrity of Antibody.)

FCM (Flow Cytometry)

(Flow Cytometric Analysis of PFA-fixed MCF-7 cells using MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

product-image-AAA23917_FCM4.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed MCF-7 cells using MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IF (Immunofluorescence)

(Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).)

product-image-AAA23917_IF3.jpg IF (Immunofluorescence) (Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Reddot (Red).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Mouse Monoclonal Antibody (MSH6/3086).)

product-image-AAA23917_IHC2.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Mouse Monoclonal Antibody (MSH6/3086).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Small Intestine stained with MSH6 Mouse Monoclonal Antibody (MSH6/3086).)

product-image-AAA23917_IHC.jpg IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Small Intestine stained with MSH6 Mouse Monoclonal Antibody (MSH6/3086).)
References
Peltomaki, P., et al. 1993. Science 260: 810-812.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
163kDa
NCBI Official Full Name
DNA mismatch repair protein Msh6 isoform 1
NCBI Official Synonym Full Names
mutS homolog 6
NCBI Official Symbol
MSH6
NCBI Official Synonym Symbols
GTBP; HSAP; p160; GTMBP; HNPCC5
NCBI Protein Information
DNA mismatch repair protein Msh6
UniProt Protein Name
DNA mismatch repair protein Msh6
UniProt Gene Name
MSH6
UniProt Synonym Gene Names
GTBP; hMSH6; GTBP; GTMBP; p160
UniProt Entry Name
MSH6_HUMAN

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Product Notes

The MSH6 msh6 (Catalog #AAA23917) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MSH6 (DNA Mismatch Repair Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MSH6 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. ELISA: For coating, order Ab without BSA FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the MSH6 msh6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MSH6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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