Mouse anti-Human MSLN / Mesothelin Monoclonal Antibody | anti-MSLN antibody

Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin

Cat. Num. AAA12374

• Gene Names: MSLN; MPF; SMRP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MSLN / Mesothelin, Antibody; Mouse Monoclonal [clone MN-1] (IgG) to Human MSLN / Mesothelin; Anti-MSLN / Mesothelin Antibody (Extracellular Domain, clone MN-1) IHC-plus; MSLN; CAK1 antigen; MPF; SMRP; Mesothelin; CAK1; Human MSLN; anti-MSLN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: MN-1
• Specificity: Cross reactivity with homologues from other sources has not been tested.
• Purity/Purification: Protein A Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 630

• Applicable Applications for anti-MSLN antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:10000 - 1:50000), IHC-P (15 ug/ml), WB (1:1000); Optimal dilution to be determined by the researcher.
• Target Species: Human
• Immunogen Description: This antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.
• Usage: This antibody has been tested for use in immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 40 kD in size corresponding to mature mesothelin by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, archival PEFF human tissues were deparaffinized followed by hydration. Antigen-retrieval is recommended. Block tissues with 1% BSA in PBS for 30 min at 23 degree C. Antibodies are diluted in 1% BSA and reacted with tissue for 60 min at room temperature.
• Immunogen Species: MSLN/Mesothelin antibody was raised against Human
• Immunogen Type: Recombinant protein
• Immunogen: MSLN / Mesothelin antibody was raised against this antibody was produced in mice by repeated immunizations with a recombinant protein corresponding to the extracellular domain of human mesothelin.
• Antigen Modification: Extracellular Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

WB (Western Blot)

(Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.)

IHC (Immunohistochemistry)

(Anti-Mesothelin Antibodies - Immunohistochemistry. Immunohistochemistry using anti-mesothelin antibodies to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission fromClin. Cancer Res. 11(16):5840-6.)

IHC (Immunohistochemistry)

(Anti-MSLN / Mesothelin antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 15 ug/ml.)

Related Product Information for anti-MSLN antibody

This gene encodes a preproprotein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Alternative splicing results in multiple transcript variants. Mesothelin is a cell surface differentiation antigen found in mesothelial cells, mesotheliomas and ovarian cancers. Although the function of mesothelin is unknown, it may play a role in cellular adhesion. Three transcript variants have been described.

NCBI and Uniprot Product Information

• NCBI GI #: 53988380
• NCBI GeneID: 10232
• NCBI Accession #: NP_037536.2
• NCBI GenBank Nucleotide #: NM_013404.4
• UniProt Accession #: Q13421; Q14859; Q4VQD5; Q96GR6; Q96KJ5; Q9BR17; Q9BTR2; Q9UCB2; Q9UK57; D3DU65
• Molecular Weight: 67,940 Da
• NCBI Official Full Name: mesothelin isoform 2 preproprotein
• NCBI Official Synonym Full Names: mesothelin
• NCBI Official Symbol: MSLN
• NCBI Official Synonym Symbols: MPF; SMRP
• NCBI Protein Information: mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
• UniProt Protein Name: Mesothelin
• UniProt Gene Name: MSLN
• UniProt Synonym Gene Names: MPF; MPF
• UniProt Entry Name: MSLN_HUMAN