SDS-PAGE
(SDS-PAGE Analysis of Purified MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).)
Rabbit MUC1/CA15-3/EMA/CD227 Monoclonal Antibody | anti-MUC1 antibody
MUC1/CA15-3/EMA/CD227 (Epithelial Marker)
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human. Others not known.
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Synonyms
MUC1/CA15-3/EMA/CD227, Antibody; MUC1/CA15-3/EMA/CD227 (Epithelial Marker); Breast carcinoma-associated antigen DF3; CA15-3; Carcinoma-associated mucin Episialin; Epithelial Membrane Antigen; H23AG; KL-6; MAM6; MUC-1; MUC-1/SEC; MUC-1/X; MUC1-alpha; MUC1-beta; MUC1-CT; MUC1-NT; MUC1/ZD; Mucin 1 cell surface associated; Mucin-1 subunit beta; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; Tumor-associated epithelial membrane antigen; anti-MUC1 antibody
Host
Rabbit
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG
Clone Number
MUC1/1887R
Specificity
This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.
Form/Format
200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
264
Applicable Applications for anti-MUC1 antibody
IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Immunogen
Human milk-fat globule membranes (HMFGM)
Cellular Localization
Cytoplasmic and Cell Surface
Hu-chromosome Location
1q22
Positive Control
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
SDS-PAGE
(SDS-PAGE Analysis of Purified MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).)
SDS-PAGE
(SDS-PAGE Analysis of Purified MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/1887R).)
References
Zotter et. al. Virchows Arch A Pathol Anat Histopathol. 1985; 406(2):237-51. Hilkens et al. Eur J Obstet Gynecol Reprod Biol. 1985 Jun; 19(6):356-7. Hilkens et al. Int J Cancer. 1984 Aug 15; 34(2):197-206.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
265-400kDa
NCBI Official Full Name
mucin-1 isoform 2
NCBI Official Synonym Full Names
mucin 1, cell surface associated
NCBI Official Symbol
MUC1
NCBI Official Synonym Symbols
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
NCBI Protein Information
mucin-1
UniProt Protein Name
Mucin-1
UniProt Gene Name
MUC1
UniProt Synonym Gene Names
PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
Similar Products
Product Notes
The MUC1 muc1 (Catalog #AAA214457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MUC1/CA15-3/EMA/CD227 (Epithelial Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's MUC1/CA15-3/EMA/CD227 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the MUC1 muc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC1/CA15-3/EMA/CD227, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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