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IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
Mouse anti-Human MUC1 / EMA /CD227 Monoclonal Antibody | anti-MUC1 antibody
MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
MUC1 / EMA /CD227, Antibody; MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody; Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen, Tumor-associated mucin; anti-MUC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
MUC1/845
Specificity
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
264
Applicable Applications for anti-MUC1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasmic and Cell Surface
Immunogen
Human milk-fat globule membranes (HMFGM)
Hu-Chromosome Location
1q22
Positive Control
MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistchemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC1 / EMA Monoclonal Antibody (MUC1/845).)
References
Rughetti, A., et al. 2005. Recombinant tumor-associated Mucin 1 glycoprotein impairs the differentiation and function of dendritic cells. J. Immunol. 174: 7764-7772.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
265-400kDa
NCBI Official Full Name
mucin-1 isoform 2
NCBI Official Synonym Full Names
mucin 1, cell surface associated
NCBI Official Symbol
MUC1
NCBI Official Synonym Symbols
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
NCBI Protein Information
mucin-1
UniProt Protein Name
Mucin-1
UniProt Gene Name
MUC1
UniProt Synonym Gene Names
PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
UniProt Entry Name
MUC1_HUMAN
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Product Notes
The MUC1 muc1 (Catalog #AAA13841) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC1 / EMA /CD227 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml) Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the MUC1 muc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC1 / EMA /CD227, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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