IHC (Immunohiostchemistry)
(MUC1/EMA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MUC1/EMA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human MUC1/EMA Polyclonal Antibody | anti-MUC1 antibody
MUC1/EMA Antibody (C-term)
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MUC1/EMA, Antibody; MUC1/EMA Antibody (C-term); Mucin-1; MUC-1; Breast carcinoma-associated antigen DF3; Cancer antigen 15-3; CA 15-3; Carcinoma-associated mucin; Episialin; H23AG; Krebs von den Lungen-6; KL-6; PEMT; Peanut-reactive urinary mucin; PUM; Polymorphic epithelial mucin; PEM; Tumor-associated epithelial membrane antigen; EMA; Tumor-associated mucin; CD227; Mucin-1 subunit alpha; MUC1-NT; MUC1-alpha; Mucin-1 subunit beta; MUC1-beta; MUC1-CT; MUC1; anti-MUC1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MUC1/EMA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1176-1205 amino acids from the C-terminal region of human MUC1/EMA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
1176-1205
Sequence Length
259
Applicable Applications for anti-MUC1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(MUC1/EMA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MUC1/EMA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MUC1/EMA Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MUC1/EMA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MUC1/EMA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MUC1/EMA antibody detected the MUC1/EMA protein (arrow).)
WB (Western Blot)
(MUC1/EMA Antibody (C-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MUC1/EMA antibody detected the MUC1/EMA protein (arrow).)
Related Product Information for anti-MUC1 antibody
This gene is a member of the mucin family and encodes a
membrane bound, glycosylated phosphoprotein. The protein is
anchored to the apical surface of many epithelia by a transmembrane
domain, with the degree of glycosylation varying with cell type. It
also includes a 20 aa variable number tandem repeat (VNTR) domain,
with the number of repeats varying from 20 to 120 in different
individuals. The protein serves a protective function by binding to
pathogens and also functions in a cell signaling capacity.
Overexpression, aberrant intracellular localization, and changes in
glycosylation of this protein have been associated with carcinomas.
Multiple alternatively spliced transcript variants that encode
different isoforms of this gene have been reported, but the
full-length nature of only some has been determined. [provided by
RefSeq].
membrane bound, glycosylated phosphoprotein. The protein is
anchored to the apical surface of many epithelia by a transmembrane
domain, with the degree of glycosylation varying with cell type. It
also includes a 20 aa variable number tandem repeat (VNTR) domain,
with the number of repeats varying from 20 to 120 in different
individuals. The protein serves a protective function by binding to
pathogens and also functions in a cell signaling capacity.
Overexpression, aberrant intracellular localization, and changes in
glycosylation of this protein have been associated with carcinomas.
Multiple alternatively spliced transcript variants that encode
different isoforms of this gene have been reported, but the
full-length nature of only some has been determined. [provided by
RefSeq].
Product Categories/Family for anti-MUC1 antibody
References
Behrens, M.E., et al. Oncogene 29(42):5667-5677(2010)
Lacunza, E., et al. Cancer Genet. Cytogenet. 201(2):102-110(2010)
Meyer, T.E., et al. PLoS Genet. 6 (8) (2010) :
Beatson, R.E., et al. Immunotherapy 2(3):305-327(2010)
Caffery, B., et al. Mol. Vis. 16, 1720-1727 (2010) :
Lacunza, E., et al. Cancer Genet. Cytogenet. 201(2):102-110(2010)
Meyer, T.E., et al. PLoS Genet. 6 (8) (2010) :
Beatson, R.E., et al. Immunotherapy 2(3):305-327(2010)
Caffery, B., et al. Mol. Vis. 16, 1720-1727 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
122102
NCBI Official Full Name
mucin-1 isoform 2
NCBI Official Synonym Full Names
mucin 1, cell surface associated
NCBI Official Symbol
MUC1
NCBI Official Synonym Symbols
EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
NCBI Protein Information
mucin-1
UniProt Protein Name
Mucin-1
UniProt Gene Name
MUC1
UniProt Synonym Gene Names
PUM; MUC-1; CA 15-3; KL-6; PUM; PEM; EMA; MUC1-NT; MUC1-alpha; MUC1-beta
UniProt Entry Name
MUC1_HUMAN
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Product Notes
The MUC1 muc1 (Catalog #AAA286789) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1176-1205. The MUC1/EMA Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MUC1/EMA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MUC1 muc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUC1/EMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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