Mouse Myc Monoclonal Antibody | anti-Myc antibody

Anti-Myc Antibody

Cat. Num. AAA60779

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot, Dot Blot, ELISA, Immunofluorescence
• Synonyms: Myc, Antibody; Anti-Myc Antibody; anti-Myc antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Myc-tag Monoclonal Antibody. Recognises over-expressed proteins containing Myc epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-Myc antibody: ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot), DB (Dot Blot), ELISA, IF (Immunofluorescence)
• Storage Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Immunogen: A synthetic peptide corresponding to residues 410-419 (EQKLISEEDL) coupled to KLH.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Application Data

Application Data

Related Product Information for anti-Myc antibody

This gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]

References

Ahn, CS et al. "Overexpression of the PP2A regulatory subunit Tap46 leads to enhanced plant growth through stimulation of the TOR signalling pathway." J. Exp. Bot. : (2014). PubMed: 25399018.
Su,, B. et al. "A Genome-Wide RNAi Screen Identifies FOXO4 as a Metastasis-Suppressor through Counteracting PI3K/AKT Signal Pathway in Prostate Cancer." Plos One 9 (7): (2014). PubMed: 24983969.
Shi, XH et al. "The expression and localization of N-myc downstream-regulated gene 1 in human trophoblasts." PLoS One 8 (9):e75473 (2013). DOI: 10.1371/journal.pone.0075473. Application: Western Blot.
Shi, XH et al. "The expression and localization of N-myc downstream-regulated gene 1 in human trophoblasts." PLoS ONE 8:e75473 (2013). PubMed: 24066183.
Zhang, A et al. "The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage." Cell Res. 23(3):340-50 (2013). DOI: 10.1038/cr.2012.164. PubMed: 23208419.
Kim, JH et al. "The Arabidopsis RING E3 ubiquitin ligase AtAIRP3, LOG2 participates in positive regulation of high-salt and drought stress responses." Plant Physiol 162:1733-49 (2013). PubMed: 23696092.
Petzold, CJ et al. "Cells determine cell density using a small protein bound to a unique tissue-specific phospholipid." PeerJ 1:e192 (2013). PubMed: 24244914.
Tattoli, I et al. "Amino Acid Starvation Induced by Invasive Bacterial Pathogens Triggers an Innate Host Defense Program." Cell Host Microbe 11 (6):563-575 (2012). DOI: 10.1016/j.chom.2012.04.012. Application: Western Blot.
Sachdeva, M et al. "Negative regulation of miR-145 by C/EBP-beta through the Akt pathway in cancer cells." Nucleic Acids Res 40(14):6683-92 (2012). DOI: 10.1093/nar/gks324. PubMed: 22495929.
Schmid, D et al. "An abundant, truncated human sulfonylurea receptor 1 splice variant has prodiabetic properties and impairs sulfonylurea action." Cell Mol Life Sci 69:129-48 (2012). PubMed: 21671119.
Liu, TT et al. "Rab GTPase regulation of retromer-mediated cargo export during endosome maturation." Mol Biol Cell 23:2505-15 (2012). PubMed: 22593205.
Rothenburg, S et al. " Characterization of a ranavirus inhibitor of the antiviral protein kinase PKR." BMC Microbiol 11:56 (2011). DOI: 10.1186/1471-2180-11-56. Application: Western Blot.
Jung, H et al. "Mest, Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation." Biochem. J. 436:263-9 (2011). PubMed: 21375506. Application: Immunoprecipitation.
Christova, T et al. "Lymphoid enhancer factor-1 mediates loading of Pax3 to a promoter harbouring lymphoid enhancer factor-1 binding sites resulting in enhancement of transcription.." Int J Biochem & Cell Biol 42 (5):630-640 (2010). DOI: 10.1007/978-1-61779-455-1_15. Application: Western Blot.
Mo, Y.Y et al. "MicroRNA-145 Suppresses Cell Invasion and Metastasis by Directly Targeting Mucin 1." Journal of Cancer Res 70 (1):278-287 (2010). DOI: 10.1158/0008-5472. Application: Western Blot.
Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1." Biochem Biophys Res Commun 396 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. PubMed: 20152813. Application: Immunoblot.
Sachdeva, M et al. "MicroRNA-145 suppresses cell invasion and metastasis by directly targeting mucin 1." Cancer Res 70(1):378-87 (2010). DOI: 10.1158/0008-5472.CAN-09-2021. PubMed: 19996288.
McPherson, VA et al. "Contributions of F-BAR and SH2 domains of Fes protein tyrosine kinase for coupling to the FcepsilonRI pathway in mast cells." Mol Cell Biol 29 (2):389-401 (2008). DOI: 10.1128/MCB.00904-08. Application: Immunostaining.
Cho, SK et al. "Arabidopsis PUB22 and PUB23 are homologous U-Box E3 ubiquitin ligases that play combinatory roles in response to drought stress." Plant Cell 20:1899-914 (2008). PubMed: 18664614.
Zhu, S et al. "MicroRNA-21 targets tumor suppressor genes in invasion and metastasis." Cell Res 18:350-9 (2008). PubMed: 18270520.
Wu, F et al. "Gam1-associated alterations of drug responsiveness through activation of apoptosis." Mol Cancer Ther 6 (6):1823-1830 (2007). PubMed: 17575111. Application: Western Blot.
Mller, I et al. "Ca2+ signaling and gene transcription in glucose-stimulated insulinoma cells." Cell Calcium 52 (2):137-51 (2012). PubMed: 22658828. Application: Immunoblot.

NCBI and Uniprot Product Information

• NCBI GI #: 40796149
• NCBI Accession #: NP_955602.1
• NCBI GenBank Nucleotide #: NP_045935.1
• NCBI Official Full Name: MYC