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WB (Western Blot)
(Western Blot analysis of over-expressed Myc-tagged protein in 293T cell lysate using Myc-Tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane1) and 1:10000 (lane 2). Each lane was loaded with 10ug of cell lysate.)
Mouse anti-All Myc-Tag Monoclonal Antibody
Myc-Tag (19C2) Mouse mAb
Reactivity
All
Applications
ELISA, Immunoprecipitation, Immunofluorescence, Western Blot
Synonyms
Myc-Tag, Antibody; Myc-Tag (19C2) Mouse mAb; anti-Myc-Tag antibody
Host
Mouse
Reactivity
All
Clonality
Monoclonal
Isotype
Mouse IgG1
Specificity
Myc-Tag (19C2) Mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.
Sequence Length
11
Applicable Applications for anti-Myc-Tag antibody
ELISA, IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot)
Source Note
This monoclonal antibody is produced by immunizing animals with a synthetic peptide (KLH-coupled) corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
WB (Western Blot)
(Western Blot analysis of over-expressed Myc-tagged protein in 293T cell lysate using Myc-Tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane1) and 1:10000 (lane 2). Each lane was loaded with 10ug of cell lysate.)
WB (Western Blot)
(Western Blot analysis of over-expressed Myc-tagged protein in 293T cell lysate using Myc-Tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane1) and 1:10000 (lane 2). Each lane was loaded with 10ug of cell lysate.)
IP (Immunoprecipitation)
(IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:200 (1), 1:500 (2), 1:200 (3).)
IP (Immunoprecipitation)
(IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dilution: 1:200 (1), 1:500 (2), 1:200 (3).)
IF (Immunofluorescence)
(IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (right, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution.)
IF (Immunofluorescence)
(IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (right, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution.)
WB (Western Blot)
(Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of cell lysate.)
WB (Western Blot)
(Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of cell lysate.)
Related Product Information for anti-Myc-Tag antibody
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
Product Categories/Family for anti-Myc-Tag antibody
References
Munro, S. and Pelham, H.R. 1984. Use of peptide tagging to detect proteins expressed from cloned genes: deletion mapping functional domains of Drosophila hsp 70. EMBO J. 3, 3087-3093.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
MYC-tag, partial
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Product Notes
The Myc-Tag (Catalog #AAA62298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Myc-Tag (19C2) Mouse mAb reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's Myc-Tag can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Myc-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myc-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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