Mouse MYELIN PROTEOLIPID PROTEIN Monoclonal Antibody | anti-PLP1 antibody

MOUSE ANTI MYELIN PROTEOLIPID PROTEIN

Cat. Num. AAA49446

• Gene Names: PLP1; PLP
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry
• Synonyms: MYELIN PROTEOLIPID PROTEIN, Antibody; MOUSE ANTI MYELIN PROTEOLIPID PROTEIN; anti-PLP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: plpc1
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1 mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-PLP1 antibody: WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Bovine
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Published customer image: NL scheme and PLP staining. (A) Type-I NL with demyelination of the whole width of the cortex (#) and adjacent WM (*). (B) Type-II intracortical lesion evolving around a vessel. (C) Type-III subpial NL. Demyelination spreads from the pial surface until cortical layer 3. Scale bars represent 500 um.From:Yao B, Hametner S, van Gelderen P, Merkle H, Chen C, et al. (2014) 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE 9(10): e108863. doi:10.1371/journal.pone.0108863)

Application Data

(Published customer image: Iron loss in NLs. PLP myelin staining (B) and iron staining (C) on two consecutive slides of tissue MS2 disclose iron loss together with myelin loss in a NL (red arrows). Higher magnification (D, E, F, G) of PLP (D, F) and iron stainings (E, G). In NAGM (D, E), iron is present in oligodendrocytes (black arrow and inset in E) and myelin sheaths. In a NL (F, G), iron is removed from the demyelinated cortical parenchyma and present in perivascular macrophages and microglia (black arrow and inset in G).From:Yao B, Hametner S, van Gelderen P, Merkle H, Chen C, et al. (2014) 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE 9(10): e108863. doi:10.1371/journal.pone.0108863)

Application Data

(Published customer image:NLs on MRI and tissue sections stained for PLP and iron. Examples Examples of NLs from tissue MS1 (A, B and C) identified on MRI magnitude (TE = 25.2 ms) and R2* images as well as color-coded PLP-staining and iron staining. Red arrows point towards NLs identified by MRI and confirmed to correspond to area of demyelination by the color-coded PLP staining. Blue arrows point towards NLs identified by the color-coded PLP staining and only retrospectively identified by MRI. Black arrows and black box point towards NLs identified by the color-coded PLP staining and not by MRI even upon a second retrospective image inspection. In the black box we include a large area of demyelination which goes entirely undetected by MRI. In the color-coded PLP staining of figure: green = complete WM demyelination, red = complete GM demyelination, yellow = areas of variably reduced myelin density.From:Yao B, Hametner S, van Gelderen P, Merkle H, Chen C, et al. (2014) 7 Tesla Magnetic Resonance Imaging to Detect Cortical Pathology in Multiple Sclerosis. PLoS ONE 9(10): e108863. doi:10.1371/journal.pone.0108863)

Related Product Information for anti-PLP1 antibody

Mouse anti myelin proteolipid protein antibody, clone plpc1 recognizes myelin proteolipid protein (PLP) in many mammalian species (Stoffel et al. 1985). Clone plpc1 also recognizes the alternative PLP splice variant lacking part of the cytoplasmic domain (amino acids 117-151), known as DM20 (Simons et al. 1987). PLP encodes the major protein components of compact CNS myelin and mutations in the PLP gene can lead to severe dysmyelinating disease (Hudson et al. 1989).Mouse anti myelin proteolipid protein, clone plpc1 has proved a useful immunohistochemical tool for the study of central nervous system injury in patients with multiple sclerosis (Seewan et al. 2011, Huizinga et al. 2011)

NCBI and Uniprot Product Information

• NCBI GI #: 68299750
• NCBI GeneID: 281410
• NCBI Accession #: NP_776574.2
• NCBI GenBank Nucleotide #: NM_174149.4
• UniProt Accession #: P04116; Q3ZBU4; Q9TSH8; A2VDT3
• Molecular Weight: 26,212 Da
• NCBI Official Full Name: myelin proteolipid protein
• NCBI Official Synonym Full Names: proteolipid protein 1
• NCBI Official Symbol: PLP1
• NCBI Official Synonym Symbols: PLP
• NCBI Protein Information: myelin proteolipid protein; lipophilin
• UniProt Protein Name: Myelin proteolipid protein
• UniProt Gene Name: PLP1
• UniProt Synonym Gene Names: PLP; PLP
• UniProt Entry Name: MYPR_BOVIN